	   SnapGene      +Aaatgaaagaccccacctgtaggtttggcaagctagcttaagtaacgccattttgcaaggcatggaaaatacataactgagaatagagaagttcagatcaaggttaggaacagagagacagcagaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagatgcggtcccgccctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcggcgcgccagtcctccgatagactgcgtcgcccgggtacccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggactcgctgatccttgggagggtctcctcagattgattgactgcccacctcgggggtctttcatttggaggttccaccgagatttggagacccctgcccagggaccaccgacccccccgccgggaggtaagctggccagcggtcgtttcgtgtctgtctctgtctttgtgcgtgtttgtgccggcatctaatgtttgcgcctgcgtctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagttctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtggcccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggtaggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaaccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtctgactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagtttgaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaagaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggccgcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacctggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttttgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctccatccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctccctttatccagccctcactccttctctaggcgccggaattcagatcttacgtagctagcggatcccaattggccaccatggaacaaaaactcatctcagaagaggatctgatgactacagtagtggtacatgtggactccaaagctgagctcactaccctgctggagcagtgggaaaaggaccatggcagtggccaggatatggtaccaatccttaccagaatgtctgaattaattgaaaaagaaactgaagaatatcgtaaaggagatccagatccatttgatgatcgacatcctggtcgagctgatccagagtgtatgctgggccatttgctgagaatactcttcaagaatgatgatttcatgaatgcgctggtaaatgcatatgtgatgacaagtagagagccccctttaaatactgcagcttgcagactgctgctggacatcatgccgggcctagaaaccgcagttgtctttcaagaaaaggaaggaattgttgaaaatcttttcaaatgggcccgagaggctgatcagccacttaggacatattctactggactgctgggaggtgctatggaaaatcaagatattgctgccaactacagagatgaaaattcacagttggtggcaatagtgcttagaagactgagggagctccagcttcaagaagtggctttgcggcaggacagtaagcgtcctagtccacggaagctctcttcggaaccccttttgcctctggatgaggaagctgtggatatggactatggtgacatggcagtggatgttgtggatggagaacaagaatcttctagagatatggagatctcctttcgtcttgattcaagtcacaagaccagtagtagagtgaactcagcaactaaacctgaggaaggaggattaaagaaaaacaagtcagcaaagcatggtgacagagagaactttaggaaggccaagcagaagttgggcttctcatcttctgacccagatcgtgtgtttgttgagctgtctaatagcagctggtcagaaatgtccccctgggtgatcggcaccaattacactctctatcctatgactcctgctattgagcagagacttattcttcagtatttgacccctttaggagagtatcaggagttacttcccatattcatgcaacttggatcaagagaattgatgatgttttatattgatctgaaacagacgaatgatgtcctgcttacttttgaggcactcaagcacctagcatctctcctgcttcataacaaatttgccacagaatttgttgcacatggtggagtacagaaattactggaaattcctcgtccttctatggctgcaactggtgtgtccatgtgcttgtattacctgtcctacaatcaggatgctatggaaagagtttgcatgcatcctcacaatgttctatctgatgtggtgaactataccctgtggttaatggaatgttctcatgcttcaggatgttgccatgctactatgtttttctcaatttgtttctcattccgggctgttttggagctctttgaccgttatgatggtcttcgtcgtctggtgaacttgatcagtactttggagattttaaatttggaagaccagggtgcacttttgagtgatgatgaaatatttgctagtcgccaaacaggaaaacatacctgcatggctctgcgcaagtattttgaggctcatctggccattaaattggaacaagtcaagcagtcacttcagaggactgagggtggtattcttgttcacccccagcctccatacaaggcatgctcatatactcatgagcagattgtagaaatgatggaatttttgatagagtatggcccagctcagctatattgggaacctgctgaagtcttcctcaagctttcctgtgtgcagcttttactacagcttatttccatcgcctgcaattggaagacctattatgcaaggaatgatactgtgcgttttgctttggatgtccttgctattctcactgtggtgccaaaaatccagctccagttggcggagtcagtggatgtactggacgaggccggctctgctgtctccactgtaggtatcagcattattttgggcgtggctgagggtgagttctttatccatgatgctgaaattcagaagtcagcacttcaaattatcatcaattgtgtatgtggtccagataaccgaatatccagtattggcaaatttatttctggaactcctcggagaaagctgtctcagactcccaagagtagtgagcatactctggccaagatgtggaatgtggtccagtccaacaatggcatcaaggtgcttctgtccctgttgtccatcaagatgcccattacagacgcagaccagatccgggccctggcctgcaaagcccttgtaggcctgtctcgaagtagcactgttcgacagatcatcagtaaacttcccctttttagtagctgccagattcaacagctgatgaaagagcctgtgctgcaggataagcgtagtgaccatgtcaagttctgcaaatacgctgctgagctcattgagcgtgtatcaggaaagcctcttctcattggcactgatgtgtctctcgcacgactgcagaaagcagatgttgttgctcagtcgaggatctccttccctgagaaagagctgctcctgttgatacggaaccatctcatttctaaagggcttggagagacagcaactgtgctgacgagagaggctgacctgcccatgactgctgcctcacattcttctgccttcaccccagtgactgctgctgcttctcctgtctctcttccccggacccctcggattgccaacggcattgcaagtcgactgggcagccatgctactgtgggtgcttctgccccttctgctccccctgcccatcctccaccacggcccccccagggttcattgcctttgcctggcccatcatatgcaggcaattcccctttgattggtaggatcagttttatcagagagaggccatcaccgtgcaatggcaggaaaattcgagtgttgcggcagaagtcggaccatggtgcatacagccagagccctgccataaaaaaacaattggatagacatcttccttccccacctacgctggacagtatcatcacagagtatcttagagagcaacatgctcgatgcaagaaccccgttgccacctgtcctcctttttctctctttactcctcatcagtgtcctgaaccaaaacagaggcggcaagcgccaataaactttacctcaaggctaaaccgcagggcatcatttccaaaatatggaggggtagatggcggatgctttgataggcaccttatctttagcagatttcgtccgatttcagtattccgggaagctaatgaagatgaaagtggcttcacctgttgtgcattctcagcccgggagcggttcctgatgcttggcacttgtacagggcagctgaagctctacaatgtgtttagtggacaggaagaggccagctataactgtcacaactcagccatcacacaccttgaaccgtcaagggatgggtccttacttctgacatctgccacttggagccagcctttgtctgcactttggggaatgaaatcagtatttgatatgaagcattccttcacagaagatcactatgttgagttcagtaagcactctcaggatcgggtcataggcacaaaaggagacattgcccatatttatgatattcagactggcaacaagctgttgactctgtttaacccagatcttgccaacaactacaagaggaactgtgccacctttaatcctacagatgatcttgtcttaaatgatggcgtcctctgggatgtccgctctgcacaggccatccacaagtttgacaagttcaacatgaatatcagtggtgttttccatccaaatgggctggaagtcatcatcaatacagagatttgggaccttcggactttccatcttttacacactgttcctgctctggatcaatgccgtgttgtttttaaccacactgggacagtgatgtatggagcaatgttgcaggcagatgatgaagatgacttactggaagagaggatgaaaagcccttttgggtcatcctttcgaacattcaacgcaactgattataaacctatagcaactattgatgtgaaacgaaacatctttgacctgtgcacagacaccaaagactgttatcttgctgtcattgagaatcaaggcagcatggatgcactgaatatggacacagtgtgcaggctctatgaagtgggcaggcagcgtctggcagaggatgaggatgaagaggaggaccaggaagaggaagaacaggaggaagaagatgatgatgaagatgatgatgatactgatgatttagatgaactagacactgaccagttgctggaggcagaactggaggaggatgacaacaatgagaatgcaggagaggatggagacaatgacttctctccctctgatgaggagctagcaaaccttctagaggagggagaggagggggaagatgaagactctgatgcagatgaggaggtggagctgatcctgggggacacagacagctcggacaactctgatttggaagatgacatcatcttatctctgaatgagtgactcgagcgggatcaattccgccccccccctaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgataataccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaagcggccgcgactctagagtcgacctgcaggcatgcaagcttcaggtagccggctaacgttaacaaccggtacctctagaactatagctagcatgcgcaaatttaaagcgctgatatcgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagcttaagtaacgccattttgcaaggcatggaaaatacataactgagaatagagaagttcagatcaaggttaggaacagagagacagcagaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagatgcggtcccgccctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcggcgcgccagtcctccgatagactgcgtcgcccgggtacccgtgtatccaataaaccctcttgcagttgcatccgacttgtggtctcgctgttccttgggagggtctcctctgagtgattgactacccgtcagcgggggtctttcatgggtaacagtttcttgaagttggagaacaacattctgagggtaggagtcgaatattaagtaatcctgactcaattagccactgttttgaatccacatactccaatactcctgaaatagttcattatggacagcgcagaaagagctggggagaattgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggcgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggcgattagtccaatttgttaaagacaggatatcagtggtccaggctctagttttgactcaacaatatcaccagctgaagcctatagagtacgagccatagataaaataaaagattttatttagtctccagaaaaagggggg  \ƒ   #   (   !   '   7   G   3   #   0         #   0   6   !   7   <   =   #   3   I   >   -   .   Q   #      3      *   )   0   0   &   "   /         "      .      ,   -         3   (   "         +   E   8   (   !   K      -   8   "   F   "   7   -   M      2   A   E   >   *   '   "   4   "   "   ;   B   (   9   C   ,   ;   7   Q   .   )   -   -   %         1   -   0   5   C   .   -         &   !   &      0         >   -   +               7   3   $      6         (      .   2   4   '   E   2   8   1   C   =   1   5       :   .      =   ?   8                  *         $      ,   )               1   2   *   .      "   $      E   &   7   .      3   9   /               C   4   .   +   G      <   6      1   ?   &            -   &                      !         (   *   6   P   ;   A   K   &   '   1   1      %   <      *   &   1   @   G   C   I               "      =   @      @   9   3   '   !      ,   C   *   5   3   K      3   3   -   0   4   T        # ÷øøù>hÿ /0N”×V#$% !¾	 v ø ¬#è&ú'è(¡)¢)*:+;+<+( Ó(=ãðÙ�ãŸ�´/iXÄö1yB•e&û¿I w ù ö!Ñ#¡$@%«&x'·(o)£)! õ ’×ù@÷f	¤.–ŠÀ?4¹\Ø»ÿ"-S
 ­#R'À'û'×(á(½)È)' € ­ úÛ�\	IŠ�Ýx'Ô¥ëOÄ!5W©ÖŒ >"¥$%é&€'Ë(é(t)�)*7 Ô>äFþbŽärŒÕ¹	Ä	g
SŒHã;µPÊ0H�œŸ¸ÁÞó÷ü#ÊÕDbRüBXÀx ¢$•(¸(<)ÿ)G > i ¿#}Ú}ª?C™)AÑ		x
Æ
›Òy êüFYÉ"‚÷jªC9?KP§ñ,IXŠ÷'g’óJ !)!¯!÷!,#½#ý$A%Ú&a'y'‰'p)ï)3 Š 4i–Â;JenØà	½
Q¢\økJ0´YïKâ!'¢½zí'Cf³ú Ü!µ"Œ#Ò#í#¬&ù&Ó'°(¤)Í*#  Ã)´ñ·àw…P'ZS>‰òÅÍñ32A–ñêG¿v)«‘!0 ö ¼‘ÉWög	d
—
ð
w¥/-—e‹E“ ²ŸV»ÙÜ ¡ €!&"Ï"å"_#†%b&S'o(x(Ø(â(!)|)ô)e*’* ” “AB)×%Ð2yô@3µÍ½¼.TÊ ß "o"‰"®"Ä"#8#Y#™%¾)  d 	<xA•Áºtœ=�€ ç b#®%ã%"&Á'ü'É)# ¼ É ØúP ¡ÅøƒT	iÜM5º]5èIåòë #µ ,"®#Ù#5$%ð%›(×*0 q±wE‹ýGN˜ÍÅõ"‚ðš} � Ã Ü B!ƒ!¢!ÿ!Ì"¾%Š& &ê&�'L(Ì(Þ(N)u)š)±)è)*O*n*w*Œ*ª*²*6  9 zxÌ_n	$
R÷]kgyâ4 (WÈÕÁë+Ú“á„Ð1Rñ6KX™Ãq¦Bb¡"G#T$~%(&e'û(! § ãfù	�
Jãød�Þ ˜¦ÿ Ê¼Ð½ P!?"ó"¦$Û$ž%ê(î(`)Ý*7 � ® I×ûÜXm®¡ž]	Œ`;ž¤·¶Óýk™®ìùªP…e†öŒª×k !$!6!è"²$%&X(‚)**›*< Õ?Àd�á?ÏÖ"
ö%,^” þû<ûI%.1=I‘� »ÜñôúýérÈE½ýQ{ ~!Â"R$£$V(°*= M T o q à åGL8s…c©êb¶s…�ýÿš	º	ç	�°äæúü	£Ë½GÑ¯¹Âø8v}˜š	CÁy ß#Ë${&	'–()=) *# È’¸¼	Å	h
Ë
°†Iš)+™ lèücS™
Y ~"Ø"3#+$I%Á%e&‘&)+3 P]íÿ‡ìåA	•	Te0DM¶S¥¶Qß$u
¾ËìÖ-(zy†¹î Æ !a!¾"%V'Ï'¹()D*R*+I V ƒ5Û½S@ÚôBÒ
		œªë·Á8ƒŒÜø�‘«5D+:@LO[	Q�FJYc$0?¥Ç2’:<ô°!ø!à"ý"#•#$þ$%B%&b'³'q)J*þ*>  ? ÓGM%:Lš»àY	
o
y
Ç
ºÓq¯Zˆ²#’oO—œòø§,h/œµÊK !†!-#„#Â#Û&í&Š'ê'('({(&)?)�)5*4+- O z ¼$jT~6>V«¸ÇD{é	
U
	zýéGk‰Óë±h¨-=(x£^„Ãf ò }&z'’'ß'ð). j ´ â ÀÏN~;«*u¼	ó
z¡Ê¹ð% iCy—‹�#•¤ã×“ÝEE	!*!K" #¾#Ã&Q ¹ Ä ë -jÈt¼ß¸<foî	ñ	
¾
ë
f´·vR©A€q�²DK�Á19KZ/28L’ã"(RUX^admv£¾ÙT(%âíQ&'r(±(3)¥)*h*•*¡*# ‹ �5 £K‡‚âÄlS|ßg¿î°ùÿ´ªû ›!Ý!“$œ$Ã$­&Ô'q*Î*+ âæ¿ŸÙ�jø]ðis¿zpK¶"y#Ó#á#â$)´)µ*"+3 ^ Ì›T…—ÃekwŒj�2	j	á	þ	€˜Ô£ù”µ	<ð){DI­Ag‡B#�#˜# #î#ú#c$¸%Ê%ø%ú&'/'x)X* ÅçI:u	
[�÷—òøëîHÀów^!q!³#(u(˜(Ø)å)* s ¸{âxZùÐèpŠóBÍ{³ò¦Ä39BWf›Šœ¬�!Ž":$„'8(…(¾(É*) …Äêò½áËá†ÝQˆ	œ	( �3tçùÎ;¼Êp¯<’®äo§ ’!“"ƒ$^&é*0  *1µ*ù‡ÀÔêI­,	¯	]Ÿî“&T?Æ§4…y9r{�üÕ/[ž0*4Ì!]"Í$�&0 ’ Áº½!:ÕÞÊ÷Hcle
î
ÿ
0+OŽÚDKˆ–ð‘
!õšÚ»é�!ý!ã"æ"`#»#%„%M*Š*Õ*& ÒÝXº÷h	ñ
x²˜.$1•nçDæ¼p	!¢YÈ !P$\&c&T'p(y($)f*“*" Yo®Á”6	8	˜
š
ÀÔQt-éé”úø³·hÃþ'"C"Ž$‡%&‚&Ù(ã(")/ ÷ ku’Ó¢
¦Â.fòŒF¥%¬úµ ÜWÝ^…ŠÉü : U »!E"Ð"¹$×%Î&#'()})¨)õ)  C*�Òç²µnv+´Äî!õAt¶:U")"É"9#Z#�$O%­* ( • B]Ñ
Ü§û…Q¾/g  à /!p"Š"¯"-$K%&M&'ª(8)¿)Þ)" š«É–©	¾	¨
ï3ˆzÖòSŽ›zv¹>Å‡ Ë "Å"###$š%„&ã'O( ””Ø&j
…UÏ4eÎ½g&)
+. °=.IÆi	>	[
Bt’•Á/À¢�öü%Š:ãêòÛ?. <!5#|$:%l%o%Ô%ü%
&“&'�'Û(l)º)Û) e šåB–Ç	aÀ“ÅuÃŽÐè ##Ì#>$Z%Ã%ä%&#&Â'Ã*,  Ù î ²Tq„ÿyÃõ:	ô	Í
/=Nþëÿw–ÿ¹j�>5� E!¥!c#k#$a%•%L'j(Ê)ä*- Y œ *;
Ê=Z©Eâ
;œÌ1Â»¢€äPÐ	BU‚ÅSdô¢¹ Ç!""0"T"Ú"q$R%¯%á&'ý' ‹Q&f„pÇ�q;Aoé›´[F R ¶ -"‹$’%å(k* 00ù¦éU	Kº,*»®^Ëþz!Ú#‰%ÿ%A&s&£&œ(Ç(Ñ(Ó)3 Ùû[_e�f�’³¹Ï¢.:1šF	js­m¼vF]B½Úÿ6p€{ì$y"¯#6$h$/%È'!(a(¦(-+( ½ Ê zêÆ^	
ƒ
œ
±
ÝNSnwÊëo6Q]ºÐJæó£ä!€"%ñ%*¦*º*Ø*" Ç N²”ÿF2þH¸wˆ¨ÎŠðt ~ Á È Ý Í"]#á%ˆ&Ñ'®(Õ(ß(ù()) qw™Æö#ñ?�Ä „!À"a$É$¿%‹&ë&‚'v)›)²)P*o*³* ‰mŽ‹	ŒOO·ƒÇ², … :!C!£!û!¡&J'M(h(Ï(6)¤*«*+ ?Artˆ‰œxËéØ=çôhj›�°1 r Ž ¯ ½! "d"##%e%¶&(f(Í(O)d)é)*<*x*�*E w ™ Ö ™¡Qþ+`	N	—	gÄ�…Œzß5$fÖ(,Ûü”µM�…‡¨�ÄËà£ ÂÿØ²!¢"¥"»"s#v#�#
%&)&À&Œ(»()8  5 ÛÍÃíÙ	‘
ëäøø©6ìmã§µì t‰åâ1ÈÀ2SòL*«éC^!!H#o#¾$ë$%g%Ù%X'f'p'f)­)ë)û)~*( : ¶{¤³×ò4
Ñ
Õ
ßS€2^hH)§„Þ(7šr§ãcÞ÷^ c!%1&7'¶'ü(H)÷)! yo	%
-
^
¨¾Çl}XÉa«Âf®æÑYJ«ÚYG"U$,&%'().)„*K ¯ÉF–žêû‰š¦ÍgŠ©EC	Q	Ž
K§j‘¬ñ>A¸ô7:†Ùó§È�&!Íî6W/_þO|öñ»[ ¬ ¾ ?!";"@"a"#V#»$'ë(a)—)ª)û*  ž�”�	Á
'Ã—‚†á•Ë½ßG Ò"ô"E%x%‹)- " .5CräÕíú	
?
¤
¸ÇÓhß=ŽN™Ä£\_Œ¯KW^l> ˜ Õ!"G$§$Ÿ%T&¢(ï(8 ¨ IR¦Lïç VäùW’<eF˜»�®ßaê =ÎÑr{×3 �   ± Ñ Q!t!³!¿!"4"#<#¶#Ô$Ü$Ž%©%è%–&Ð&�(>*Þ*" :wØü†nza÷š ¯×’‡Ò_b�¹ð7!Ò!ú$%=%&m'´(2*�*˜*F - ‚ ¯ vJÝY¢Ÿo¬	O
"Ê0<ôa}OZÃþIS'l&«²Þ(¾s²V«ØŸyW l !%!›"¨"#ö$V%r%F&¸&(Q)X)“)Ä)*$*z*œ*+" ¨Š`ÞO 5Fà¥¸^%ÉÏW¢Q<  è!f"é"$$%ƒ) *+7 ¢ Ï ù _Žî#¯þ)eî^�ƒŸ®	”¦·ÔU°ÓT…íú+†ßZ`fÃ÷#Ëø"ˆ·: V!Y"³$œ&Y(Ã(*F*T*- ¬ ô Ö'ïÚâ£-•Ü¿&=Ó.õþêÃ 4"ÕþR÷ Ð#¤$ %w''¿'ù'Ê(s) )Ç)9+M h ‰ ÿ 3EÁa>Bv@ÐÔ×	¸	Ã	¼
Ñ&ûX¡÷!ö³ÉJ &/28>GJž¡·¼Ýòû+H&ÉÔéCF¾‘²(W!(!�!´"‹#ì#ø&`';)î)þ)Ì*  c @ÿ” Ä�xd4ž2s ´<Œ!"+"Ã"2 8 ve	#
÷Q_¶ÿáüVüÀj*Ùþ’­ƒÌ„É¥a| Û !#!5!O!¡!Ë"S$}%½%d'K(W(M)_)°)v*±*Ü*A S n p ß \7¿‘éëcµ„·üþ™	»	æ	Ê
$…(
µåûý
R˜¢¤úú¼FH-<ºðùû˜|—™…¸z }!½"×"2#H%))¯*+E N U y ³ á M4S¼5LUª·$9Ùótzß 		X	è	T
n
p¶‡±7¸�ÒBN–°/‘”â9Öw~¢Ü
D´Âñ !ü"ÿ"ƒ#”#%&|&Â&é'&(>)3+> ] ¸ Ã ê á,š„dj¢·†Ž1	Žiá@—Ó÷ŽChŽ“8¾.7Q]uS¾¬
†áìš!x#—#à#ù#’$á$P&
')2)*+!+*  r „æHà9t†¿Ü¬›	
žíŒ’çoÌæ²»~Ã8eû./›­�"Ì$—(½(È*' Ô“Òöb¹±N-#0,è•ï�÷	mÛ‰ý â"º#O$¸$%&�&Í&()L*Ô*" ' Ñæ	±´¨	½	i
„î‡ñ*ÃÛí�šyu¦d„Pf#"$,$J%N%&f&	+4 X › Ø )™ÉYô-Å	Æ	Ì
A›0ý¡v‰OéñAT�ÄRÚ4>¡!"Ù""#4#$=${$Q%Y%`%Â%	&’&'¹)ã*" y%¥èÆ¬J¹Rœp:­ýn¹š¢ZE y!".%r&Ò)*¹*,+" M›“N·vv‡×Æ<‰ó¯Ç ú!¿"\#`$%à%I'Ð'­(Ô(;*; v ˜ Õ £ Âìñ	M	–	,
3
f½1Æ‹ë¦eƒªˆÝäL¿§ªŽß)âŸÁþö!!b!¤"º"r#u#ê$+&¿&6'W'µ'º()}*B  ! QE�¥�éîˆ æìDB	P	U‘–·ÆÒ;ð E·…ØÞòà%<[Ìí5V.^Í{®ÞFJzº Z Ð ²!":";#D%¨%ç%Ž(¡(–)ú*( ^§…ÝýdínE­]|NT¯RVSŸ±‘r¸‡xï X"§"#õ$ù$Â(E*S* ++9 7 b þ Ÿ`=AÓ	PúcÛ-ÈØ¬*3GË%1ÂÈè3BŸ!`‹'Ú !"!4!Š#Ï#ë#ÿ$¼%÷&c'v'~':)r)Ÿ)¯)í)ý)8+C  ² [
36™¾KT¶8¡Ûòy«Þå	É
�ì'?~	´·�ù’6Eº,;AMP\
Rd½úú“«-	.Û„·!™!Œ"þ"1#“#–#O&%(Ç* +, & W óåõ¤ç	í«¸Â/o9”u~¬@¸ƒO€=eüx!á"$!$%M%&&q&Ì&(¸)K*;  — ¦D„ÜüèCð	+
¶¬\Š„Ý‚©ñRžKZ°4U¦]�;ˆ­òEÀõ ±!ù!9"#q#C%ß%´'¬(Á(:*ÿ*7  Z¥	ç
J_<ÝïÈ
«[‰³Ú“ÇQ¹)¯Êù,‡ñ -ƒ¶û Ù !!!!3!˜!0#‰#ê# $Þ%Ë&u'(ž)Æ*+7+Q @ YTÔ¯°	HI;M›¼áâÔ34$_`abõ4ÆP®xyz{¨©i‚! !1!2!j!k!.#/#…#†#‡#ˆ#Ã#è#é#$	$Û%Ü%Ý%Ê&Ü&î&Z'['h'r's't'ë'ì'(E(F(|(@)à)++5+6+. A ÕÓ÷NXœ½Z	I
p
z
•»r5°Åú8%5pG¡ëj0�Ël!$
$$i'‹'í'õ'G(')A)+) … U±N&Éã
ÍÕÞcö€˜�çói|ªý®ÎL ‡!J#N#Ä#/$ð$Ý&ï&\'ñ'((}(á)6*-   �U7¹ÈÔY[	
ß

sþHZ‰ælH¢ô"¬²Çiä.>)1 c $D$À$j'Œ'à'ñ)- B P { V¢W¬øÏJ	¼ê9
6Š·ì©³ky¤Ë_“žâ í$]%~&¦&°&{'“'ª'î'ö'H(()B)ñ*+% kÖ�?CE¾t	ê	J
V
q
{
8–	{æ±ÆÐûå&gìtÄÌÝ( $6%5&¾* %½%+O�|¸
6q0ÔNi…g ó m!$$K$ * cdq™�m}þx–5ÀSo þ ¿#i%µ%Þ&Á)â)1 k † µ ÐV¬vä
F
ÎÖWýd©÷î„jžD©j«No·äÆ”¯Þ1FIç
!±"#K#]'ò'*7*î*- I ¡Á²O¯½Å	Û	“
ô
{ßî!v‹z˜Eôi¥ØrF r"í"Å#0$–$%Ä&ð&+'ó(h)0 ã !eÝO!\�<'+Ê¢åËºñ &�èôŒÕŽ$ÉJŽÏM +!ˆ!L"„"O#ñ$m&')(>(~(€*5 < ~ º Vkµ¨#Z—NU]pŠôb
‹
Ä
gáwŠ[C9E2L£àï¿@¹„Gte§ã+©œ% &&&ò)*C  	Éuº½Åà¹=†	ï	
dµ¸ctÿ§Š�mÝ:I‡039M#)SVY_benw¤­ÈÚUj£*2 d Ý#$)$Á$È%Í'6(s(B*. ì ‚8.Ü8Ûg\	ò	
 
à
ì
-SØBI*r�ç›[#“Ãå?f!Ç"Ò%'�'á'¦)i*- Å ˜ÉÕ×	¿
Ó
%Uöª�€Ý¬³LÂõä³/)]&Á©åîE$D&R&†&k'Š(²(÷()4)ù)–*¢* Q ‚ÐK	ƒmT¾†ïÜz«ŸM!
"y$^%&½&÷'I(t*++ G W£D
ã]~ª»± p€Ìùü –!”$î$3&®&|'ï'C(++& | Œ 6!XÙ{¤LˆÑ	ù	½[šëÛ7}×à‹íÁÒú¥µ`%$'$«'Ù')r*Ï*ò*! C ­ùø
ÙL]4:ÅJ¸hÀ´lŒ”êã œ!Þ!�$Ä$§&±&”'š'Õ'))C)**& ãçžDF¦’	ë	û	
kœçÑôJh^jÆñhÐ�ÎÔ;ÅÍ) ‰ 7%u%×&°'Å') ×@Úu	r
ª
ó‚Ç‡{bu¦% ` ·"z#â#$3%­'-(0(;(Ž)µ)¶*0 0 lËp¢À«	K
W
9�—¿ñ|×…ü Ô.jtxÙÀqLÈíYA#Ô#l$ã$¸'Û'å'(¿*#+(+ œ€}¿ ¡	Ó	|
D5"²ùæ'|©†âm€LÞ¤%6&v&T)[) ¾¦ÏZx3	k	Ý¶*9ºíüh n!v"˜"�%ù%y)b*> _ ç &�œkžÀ	â	¹
�|ÊÍ`{ÕØUØú�“å+r•�±´ñÕZ|�œBhˆOÍ ô e!å#$L$ç$¹%&Ô&¼'3(Q(þ(J)Ñ*ô*- Ž }­†·ÄP}�%	ÿ	6
ç
ý¤Ïs�_E
=Ö¡Ã]bEJFß·"#Ž#¡#ï#$[$d$®$û&0'9'Y*+ ¸Í&%U`˜,fl~��	×
7£í™ª1o�a*ãQ®Çàj†Óë k"C#™#û#u$ %*%Ë%I&' vÇe´’óÿ/€ïI—Áôå ñ"ª#Ÿ$©&‡'v(™(æ)* ÆJ

\øš®­ì6xì_!I"‘"À#š$¶%T(Ö)ã)Ÿ*+ è;d‹‘nµá~˜ùÎyÁÊ#j%ß&(Ù) + Ž<„rTp ÿ r!"}#´#Ò$Œ%‘)Â)0*7 l t ‡ ¶ WÏ³Ãú
R
l
ÏÐŠÑþéL‹–øÑôŽ6hº°õE6:œp•�°ß2­ï Ž!²"Õ"#;${%^'Ÿ(0)*Ê*3 c­ãw©åG
¶
Ý
NXeäÄïs|§ªÅCgkÓø[gmv‹µ	�ÇŒGÛåh!L#ó'¿(8*ï*$ E ÍÚ|y[`
×qªCÎ…kŸX[d¡O¸EnJŠè# !Æ%»&×'(9(ˆ( Ñ¹x	Ýß	´ó€4Â¬åã�"÷"…'†(6 J †ëÂ¾âPû	‚‡Þu
”
)(WsÉn‚ï4u…è"á@w½èj‚™–s¯|7 ¤ ž"«".&>&_&,')i) –¢Ìó°RÆõ
*Í˜Œ{õjÙÙG“!s"î"ù"—$ô( ór	�	Ê	Ü	
|‡ªÀàË<LcÏ<Ë°=¦å”"1$%Å&@'( mÅëk³â¯¾z	‰	'
_	!6Þ­úFqk“–pŽ– ¨ Õ Æ#„$&i&ñ&'–'œ'ê* .€‚(^æ!'ÔO%\Ê,!Ù!^"S#Æ$Ø$Ž&³& '+)E)�*. ä "+è"Á¥(2®q	 ”'Ì±@ò¶5†Ï�¶LsžÜÖÜKž!P#A$«$ò$n&F'*()'*÷*2  òPúE]{ˆªÕ=Ë/
­
Û£ï€ÆZž *Ç‚¨éõ”Ä+|ŸÐ6+N à!&#õ#W$(?((†*4  f–2¶ÓÞ+�»ë	Jö,-	°	ú
?+U»z�Ö6:jv˜ýç015�¿Ð‰!Í!M"…"Î$''',*'  Òâ÷?Øöœ[Œ	e	ž‰w‰3gW¤Ã[.AMU¨ú,½ ‹ ="Q'n)¼)E = Â¾"|¶©ßý„˜:IOdmq‹ß	f
w
Å
R‹šxéPYEâjˆ:ñO©îBáð�›À"O¦WìöðaQufAª®!¼#Ù&2 “ » »;AÖ$‚ÏÈVõS	c
–
ï
 hv1,ÛLóD’›ºÛçH¼äê­"ä"
#7#X#a#Ø#4$%…%˜%!&a&ó)Ö*8  H°Wl­â Ëø^Œ
â ‹D�ö:\£jÒF—ÇêM˜àø—d…AA!‚!þ! "ç"±$�%&'&Ý(í(™)*N*‹*1 L ì¨à>Î@	”	!
¯õ+d“™/CL¯ŸÐ$®Ûkèç½'u	P  !}"Þ#*$Q$d&z&'U'Î')C*C  ÎiÒ:=FRÆKº»
ò
y¹©®ï‹‘Û$n€ˆ4N–ê*Zxg<Eb#¦>¤
"1£+.D;ƒ›Ée ß"#$‘'²'Þ'z(%)ý*= ËåÇsv‹»¾Þiži	í	ð	ý	
ê
e³¶u� ¨pÃïRÀ{Þ;J1y‘TW`cfloØ(JHøA#Ÿ#Â$É%.'q(g*”*1 
éÄ¼øÓéÊáYø+	‡	®	\%ö2Aøz:¦„¥ÉVo8qzZ3n¦ ]!²#9$‚$]&(7(t(„(è*5 ‘ jt 9­ÀÝÜGk7	™
¡
í
þ
¿Á*ÓÙCJsñ‡¤è$”«ù´æåôgº9èÇ!B"D"Ö%[&#)§)‰*   “ªÈ•§
mTuÕ³RÎs¸=~9Äÿ"("È"�$ƒ&'â'©(
)Ý): í :±pƒ¯ÂäHh9	=	ó	Z
á
.<M:sË‘”À.¡êœõþ•ãûþ$¿Ä9iÏcó~ÏÆ!j#9%n%Ó%&'Ž'Ú(k)Â*. Z^d‘²¸Î/90E	]	
›
°
lmv»+uEÉê
\A\Ê¼þ@Q ã!g$ˆ%þ%@&Ç'¥(ä(j* Æ ˆl‡�Êþè1¶§æï±+ 0 „ ® À ¼!c"#d%‡&ø()5)c)~)£*= 4 µ˜ PÖýØ	]
�
Ð
Ô
§ÞÃã÷¨5„lÞG´¦`e'­åû´0€ÇXI†Ê¢ª]×Ý] F"n#½$%Ø%0&$'‹()¬)ö)ú)? H®q•Kú™¥‰“¨ÔŽ	
>
£
À
&ÂV¦i«g@—ºó69<��œ­MÃéÇŒ^‹ý Nq>!Ñ"#F$º$w%S&•&Ï&©)8 ¡ ø í"v«4N
!/‚ó$`“¥ÈÎYÂHÒ&öÖ¡Ý*Þ'Y_ÂÊ!; 9 V Ñ!~$<%q%E&›&l'(³(#*—*  g DÙþuu	·	wµöU¿ƒ;�VN!"*"Ê"ø'J(u* R Þ ƒ+è#Øƒ¶o„n¡Bt}ï—Õ{¬û"ø#‘$G%è'®*++ ˜ÓÑ³Å,ì"îðm z$P%_%€&¸*â*++   œ¤Ž’L	2
Åïd‡ãu·ÝáIw"¹":#[#æ%¾& % – Cò˜¨«¼N¿d‹"Î#L%&N&}'$(«(9)·)* X¤S^ÒöÈÜ”ÝÙ^Fwü†ð�Íú 0!—!$$$$.$ï$É&ð'D(ß)Å*++ €BI	ˆ/²h ' b á J$5%\%4&¯& ) H   b€E
ä�¿ÅRqI0 ý q"°"ì"•$'=(ò(À)$ } 7"—Ú–MT‰…	Š
Ã
,c‰õ×Ü8~šÜîÂ?¦¨Æ"($›%%&…&ö() ø	÷
\™}…Àº~Û•!$$&$B(s*+, ›Ê|¥‘	 	ª	Ò	ú	©
¾ð4›Öò!„óÿ{wØáŽÇÓ@aˆ #k$2%¬'¯'Ä'Ú'ä'/(�)'+) � ¬_Y€	¿	æ
{¢ÉìT�œ^ŒÂa€û¶ÆÒÌ ê "j"u"—"#ä#Z$æ$%&»'2(P(Ð*ó* Š;Æƒ.Íëä ð"|#©#™$ž$¨&�)+ D ÌÎÙ²w	k
µ
MÏ‰KÐã5¹ÜÁc÷fl€mÔ"ö"z%Å%Ö' •®ú�É	
t
)†^ÌV5„Ý;?i•�•=&h&?'•'›') •çDõ'1¬
ù
Ú5µ—ÛÏ¾�!Ø!ß!%#@$ª$Å$²&ÿ&*)D)+*1  Ñö{>áŸÇ	Þ	QŠ™�èCˆõiáòiÆNfÂZN¥ßë@ù@Š 	#6#×#3$—%&Ø&Ü(m)»)2 äè÷è9ErJ�?	“	­	ì	ü	 
[~®ô€KÂîQ#zž÷y�¥Ï;a¥C:‚|"Þ"@#ž#'�'±'Ý'ç** is�±Í/Gj<	Y
¦
;9lÒu²“£Í[@ý»äó<çÆÎi#8%m%Õ%ý%Æ'¤(	)Ü)Á*. 3 ­´†p¤§ý3M
\
Ï
 Á–_kÇÍ0ÁÑ&Æ‹Õ\ÖÜ* / ƒ =!m#}$;%c%p%v%”&š& f ›Øýt×	¶	ë císl�v¸"å%ç'·**+" $ ña—AõÛ“.EvÄïMc& a Í#$$$I$4%[%È&<(ñ(¶)Ä*$ ^Ÿ	÷	bÕôƒ¡Èþ]Û|Á`Æ?§Ñé i"–"ã#å$%&$&®'º'Ã'.(1(&+ æC‰0v	È	
s
«
ËˆÜ:Û>”b×!$#{#?$©$Ä%<&þ&>'�)E  2 ³hr…õozÌöçq£ü2;	¬	
L
X
Î
:PZ‰˜­Àó‡ÌíPZeyxÁY¤MºÞ~¤?[B�‚ #h#l#�#Ö#b%–%Ü'À*æ*& `~]ô	µ	ö	’Ê ýê ÛbìÚ -Úrakî $$ä$%Ç&='¹'æ'%+)+7 1  mn¥æS1õ	
01O‘¬ò}~É†èéÿÑÒ—Ù XÉÝžZ€F!G!d#e#f#g#¤#Õ#ÿ#$V&<'¤'¥'Ë)å*$+. Ú ï þUtÄ¦Á4T–2>ºØÕÓ/ku˜ÑeÊŸ'6@ H!¦!ë!"¥#m$W&B'M'¦'	(](k(Ì) +<VuF&—mÍ(lÊfVTeº ó!#"1")#n$S%í%å&'^(l(U)ˆ)3 Z ÿ§�³Â5UÀï¡¥	Ô	A
!<³Âú(Ãv›‹£ã�nÑ
CŽµå?¨±ƒ(£U"ò#r$¡%¥%3'C'§'\)9 Û 07EË>ªÅHp~€ 	(	¢	‡
ã
3?6#�j2cÖ!?�™Ôª>Íì^²âY`n7Mì!"Û"¦#7&X&w&â&N'/ � ð �[Ì	}
E°»ÙâªMçvz0}¼‡™Òå�ËQ ÆõßA ” š I!§!È!°% 'þ'
(Í)\* oõv'g'l	Z<·Ë?Tîo˜G i ³ » ô!$"*#î%æ&m(æ(z)c*l* GRy…¿Îžr)°gspê+:»œý\o!' ¿RW§ÐC[˜4	�
nqmBnWS · ¹!."w"o$Œ$‚%�%“%ú%!'_( ,3=ŒÇÈÞ{$ÐU\fµïž ª 2"™"T%d(V)‰)C [ ª Ó è 'ß1‚ºlÀêV	¦	Á	º
‚"”L}´»ÙVšûæw­s¤±äŽ�²„ÓüPÎ õ {!"V"¸#M$^$è$¦% &4'½'È(Ò(ÿ(K)])Ò*4 ` T1�ú´6VŸð¢ã	)
6´ÃÎa|û-+‚µòoÖ}ÿ¶æ@�©‰}r× v!‘#æ#$º%k&¤&õ&¨'ì*.  ‹µÃ#	Õ	B
BûYaËÖHfÒÄÙ‘ã,¼¯²_Ì[D‚)¤"Û#s$¢%&B&t&Ò&Õ&˜'Ë'4(@*+ kŽ¨iñÁ§Û
=&)”œŒ–ÒCi”Ù! 5 ¢ Ó f!"È#ó#Ö$Š%&D'R(�(Ô)õ*G � 8F\b‘¨®Ì?g“«¶ºÆÐINÅ;8)	 
è
tæþg8k3"‘š?F^üUcìÖHe7Uq¥FKG¸aoN##°#7$e$¯$Y&x&O'‚(§( Ü ~s�G	~	ˆ
ä
×áí¾�|•Ó§#$i$0%"(à*< «üfŽ£‡±2Q$~›&	£	Xk7®žaKtžÙ`>C×¢Ä^Û _³àãÔ%8’ z"Ü">#¢#ð#:&˜&ã&ü&1':'ž'†)Z*6 1Ú`´/1q¸"‘!	|	7
9
4@$n½dwy¥Ð@GIÕ×«¯ÎÐc"mZíÁ!í!""�#\$8&ó&É'b(.+ ~¹'c&Fšp~^‚»¯ §áÔ• S!É!å!l"D#!%«%õ%J&ÿ'§*1 ñ "aägmŽé�
IÚ8>p¤ÞîOTx®1pšžÓÌÑÈ‡�àB ì J!6"�"v$Þ$'%+%'»*Ù*0+? ž {ÎÜ@Vm-“‚	‹	
;
~
„
²
Ø
±¼§½B«wËÎàì½7ˆ2Ræb+R
¡äRKÇ¤ök˜› Ã!š#ü#%ê%ò%¡'& ¾ Ë Kžë\íÇò3Y™_	Í	#oã«NŸè{Òçôt¨!µ!ï!†$±%Ì%(‘(Î)*]* ‘ö<Hœ€³¸x“ÖdºH u õ!«# $?%ª&ç&¶(Ö(à(ç(€)* ³h|•(GÈR[=IÌ@ð‰òÛ!+#ü$ˆ'Ò'”(¯( È Ow(¹±UÌñ ´ É Þ æ %"n"ˆ"Î"^#­%â%ï%n(w(š( ){)d*‘*- ¦ pyÖw m	cfµ3ÿ©Ïô 0Q�ðJ˜Âïõp‹»Ï™j ¼ Â ò"F#Ú$‰&Ÿ&ú(ç)*m*š*©*& OÇKr„†a]Ÿù*t	qÇë,¼7yxíÅ `!Á"Ê$%À%�&U(Q*+ »‚
èÀh›œŒÆ]…!J"Á#ì&(œ)4*I* €S±r¯$@þ©b$›$·%÷%'w)³)W*p* *´*  0)HzÏ€sxš÷œ®í;‰Ôò‘�ãp!Œ!Ë!\"’"Œ&ƒ'×)ä) ÀXn¹Ñ5	�PùC¶™]o„ÈûXÂT º!ü!�$ƒ%"' ™Œ\ ú† .!L&N(7)¬* Sþ¨<D`
¿’¸âÂ- ¸ ;!D!¤!/"S"Ë#p$k%”%û%à&K'i(Ú)! Š�é/eŒe-™‚
orn)PÙoÏz³x"Š$‘%¢& (`(Æ(Ð(¥* >@sˆŠp >�giœq s 9!~#È$µ&(e(g(Î( Ú²*ê�|#sÙ'è± !€#	%f%&o'(-)G)e)ê)ƒ*( -4BÈ�Ì=…`¢”õV]kðÖ2 = � — Ÿ « ° s!¾!Ô!""3"`"#U#µ#Ó$�%'Š)=** , Î u�9‰_(NyÉß„%½±Ñ"^aU÷ž¶U!ç!e"š"#%U%·&P)W)’)Ã)*1*y*Ž*6 ˆ « ó &î2“ÃÂ	»
¢ÐW¾à %Òõ²i)ý7F¶‚�Ó¤³±Ôö !'!�! !³"|%_'¾'É(L)^)Æ)Ë*Û*P \ m x · Â é àßR¾ƒ»4i�´û	0	W	˜	
S
m
#hµà‹‘–Òö†�6Q—ùg�å7»-±•7.�á…–¡àë³ð |!¼"Ö"w#‚#à$%&Á&¼( ))1)2+; š × (xX$Ð,Äa°§	ƒ@MðQÚ›/üŒçxt¥�öˆNÚˆÃ Q¡Ù3D  "#!#¹#<$N$·$-%X%&'Ñ)*Ó*A u Ô Lš¢cÿmëÁëO	D�•ÅÑ:~¼ÿê{M®× UÜ;†�©qÅÌÞžý®Y Ï !W"£"¦"t#_$é$ô$ø$§%*&5'H'�( (Ó(•)|*ù*K  6 a ± ý 2žûµä7 ñx£ªæ	ä	*
ìOµù&>b}.n¶,ðt}×2Á3T·çùA\Œžª‚¬D_ŠÚ&w!8"p#’#»%p&ö&À(®)ì)ü)9*& „ ®æWîH
Ì2Äùª7ô3Å ¸æ(h­É +ê­Ø !!i!I#M#ç#Ú%Y'g'q'ô'' $U¡œ*Îs	·
Þ
7åÏYäf	å¶ó!«Æ~ãM~Ês’Ü$C$¿$ì$¥&©'½*ð*1 dÜ [®ÄÚ	’
Vüíc¨íƒpuŠ¨DlÔóMhnw¶
4È�Hæn ƒ"%h%´%l&*'g)**í*1 ; ´§ÛÄ\óÖ	a
Ÿ
Ò
$àTb¦)B«†ß\e¸¢ÀƒFs¨äd*!Ü#Ç%Ñ%&C&Ì'5(‰(ø)A* F ØzÐ	C
ØKZ3ÚI\½ÖÑo‹éøL!	"x$2&¼&™'Ø'))*+% / o¡C�†IgÓiÅ-¨…ÏlÍ:XK¥$ _ t%£%u&Ö&·'(,(:(S)Z)< æ ·|¥ŒÎ$¶}$	5
Ö
üÜÌ_z×Î©×r’ä*Dnœ°³Õ` Y\âEÞ)8P›¹ìßd!t$­$)%€%H&Ó&8'ý(I)a* ~³´à‘­¬"H"�"É#Ñ$‹%†'S(Õ)*/*ž** bÂ¨Q
_
Ü
•�ÃgrÞ¯ô5ÒZ Zu´D‹‰Úâä" î g!º&(‡(ž(/)& lêjòqy	&
©'r¿ÈmÊKb—¬à
ç�Øj•{6 £ Ô �"ª"ø"&-&'1  ñ-Òz�©º¤p	.
>*°ÅY�)µÎ“ÃÓ*KNiuÛæ5R#ô#V$×$&'E'()&*…*ö*@ G¯á@¬×õü�›Îƒ‹9R	d	v
•
uÿX9¢vÑ‡éV¨íAŽš-—÷V–LæïcT`P¼ @!­!<"Ÿ"¬"W#°$`&P'ì(˜)G K ÊÃÍh»ÑÆë<QŠ§hßÍ*	
é
*¨’­Bo®õî¿Ú@xékƒ×'If"G7=y0&÷t-Omš¥ \!#±#8$�$y&-'(ƒ()ü*C  � 9°]c’©¬·¿ÇãFD	¯
ý
L¾)ktlrºÊ¿ðD†È 	ôý#âª¾Éý8rÎ}8¹8bò}P Å!â!"A"f$?&Z&'¨(j)ˆ*I   G‡ì!Œ”—ŸJOùü
ˆª�	
=
�
Ýç¥âh§ò4?ƒ¹#58;’›¤XG_dè¦ÆHXÿM¡Ép°8 \ ­ ¿ Ð!b"##¼$%/&b)«)"*  Ý —£t‘"‚1
Ä|âî‚–ÔàHú"÷#F%á* ŸRÇH	Ø‡Ž¾H  ë"$#( •S	„	�	‰
Â
å
ö
+˜ñ ™„�¿}Ú”!t"Y$j$1%A(õ(Œ)+ ËÍô€±´
(Îƒ‚â4-~–ÌökÚÓ"ï"õ"¨#˜$y%" ¬Ð�à8œÝ	¥
}8±àÅÌ"M?Å¤`Õæø9{"Ý"?#2$™&£( # ðüˆ/sÖž	
ÔŸ\9D=_L>Ö!h"•"H$¨$;&ý&ð(= ýgôs_}¤å\ó3Rû0•	´	
Yˆ«¹ÈŸçËìÐÚOaYd,ÐØWd£Ü}`£&? ê!"œ#£#þ#%U&Æ&;'A'£'\(@ /6DGo²î%	'	¤	Ë	@
†
l ¯Áiáb© '>L�˜uyš=ŠÉÌë]�±´áä>§°X_m“ ™ ò!(#ñ# %ì%ä&2'Ÿ'‡)[* 2nÆôBQ€
¾zY¯>r#SmÏn[—î� © ² ¸!�% 'c(@ © Ò SjÞŠh´¹ð¿"	(
Ú
5AúX`“³%Ge™Ñâ¬°Òû|“qØ  4 ¡ Ò Ö u!"""�#·#Ç#]$Õ$&j&Ñ&ô&—'Ê'?*ë*9 ªa§µM0r°!#7{	}	8
Wåf7`xHJÖØà®ûTbëÏÕGd!T¤l”ÒÓ‘ À!#=#9&—&ò&�'�(…)ß*3 J}Ûbìñ!2?Xlãè’Š	:
H=o
"¦¼Až­ÍßÑ	s¦�—R!´!Â!î!5"…$Ý$&%ª%é%ô%�(/+' ¥ �;xÕ{Gb›Q²°ïÕPcºËÎ¹ñÚ!m"‡"E#Ù$û$>%¬%ž&“(µ(*�*™*¨*! Nº/
(�ƒ`çq›‹›ÅˆÓw¨â‹!Ê!["%ö%�&(3*H*V* ˜¸ý.d,_¾(øØ\úÁ-!R"‰$K&Å(, Í 8Ù^‡o±'M)"_ƒ¡“¼ÿ&°!]`ŽöÕ< – 8!T!Ó!æ!_"T#Ç$"%&´&'n' (,)F)‚*C Á ò w%KíÞ#’½Âb3h�ê¯/	¡C�9?ßïP•õ.½PÿT6‡Ù-ÒÝê C X !&!Ÿ! #¶$ß$ó$÷$,%W%'G'”)Å)Ð){*Ú*ø*1+* ƒ ° ü # Z›å)­	r	Ë1%=Ubó2Ÿ·'¬3¬Ù%LÉ‘m 7"‚"B$³%o&)'
*¼*5 . å #¦n Ï	ž
#JÛ¥y…(Í~¨ÖA[Õm›ÔÐ9·á¿ÝëWK!w$¬$(%s%Ð%G&+()R)Y)(*`*+3  að,é£pP
q¯²~ÄJ(qÍ’³ß�)MhtŸt³×4Šzˆáí œ"©"Q#Ð$¹&'(*%*.*�*K  Ÿ  ‹à«Öôø€Ìc	Œ	
<
®
ü
Ü¤½áq¡¦¹­¾C£ÐWÇíŸ¾ç©,öü•Åå<bxS%L 7È,7l™ O [!¬!Ä!Ï!á!€$%(!*‡* QÊ!ƒ	0
³
ð�3ƒ�,} ê"ö#X$@(+3 ÏïûF^|ß 7[n.ò/”³	…
°æ¨&—ÊëxÏ[!8X‰+ÈVcê|¢=÷é!ñ!g"'#›#ý#ë%¢'[(3 Ñ i|©Ýóa‰ð >APWg6
Ù
G²_X¬ÌáSÔ S£RlÑú’¥Òœ ·!%%ó%€(„)- ¤ Ì M�—7·Ô	-]°+L_P½±îŠ[šÄþùÍõv¸À; Š!Q"Z"†"ˆ$�&(’(Ä(*G*U*0 
 À û `Ÿï$ì‘¼š.	Î	BŽ$„
-§¼Õ}Ô‘Ø,8àgê23é$‰µ$Ï$²%Ï%'('Ï)	*-*_*4 Ð hßîÉïóÿ
6Zf÷ñ.b	²	û
p ¯å¬W–¢êVUÓŒû{;akw$ù6‘ÑZ!«!¶!Î!ð!Z(T 	 £ ¿ ú Lg3456,Èìíî*45Kïð-`	a	±	@Aä,OV• ¡¸¹º»é|¼†‡ˆ‰Š‹Ž��×7‡ˆ‰™èéø12Ìè#u�W!X!Y!©!ª!N"O"P"‡$´$Í%Î%(**^*  9J  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                       A                                                                                                                                                                                                                                                                                                        )                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               	                                                                                                          6                           5                  	                                                               
         8   	                                        	                                       9                                                                
                            (   2                                 <   8                              	   !   '            
               5                           	            
            ,   "      B   +      3   7   '   1   Â   ƒ      =   %   3   <   "                        A                                                                                                                                      )                                                                                                                                                                                                                                                                                             	                                    6         5      	                       
   8 	              	             9                      
          ( 2           < 8          	 ! '    
     5         	    
    , "  B +  3 7 ' 1 Â ƒ  = % 3 < "     ¥v  §Ž  §Ä  ¨>  ¯n  ·®  Á0{’q!¾(=2‡9_
ôà ¤ ¥ À hºÏïð 458
o¼îïnÌá?=•›¸Êë2±6‡êØ{�9‹\ÿ]4ÍÎé‘â÷m !-!Ó!ê"’(**1+ùäƒ$'m*Óiß^´ Ý4š ê/ˆÃ¦"�…*À&®JœÛ&¿¼5Œ&¶§žy› ]!y^¢ú$X)'^ewleweeÆx ™ Â)nÌê™ I^ùa¥F”�þ§Öš™¢ÈJWƒŒÂr² 1 … È :!v#ú$)H)O)X)v)o*„*�*›P
Â�	‚ŸùIÐZÎäƒ$Í$É*ù…ÐM	Îäƒ$É*ùÐÎäƒ$É*D\Ð	'_zm*ÖœÓi›€%&†'%Â&!�«¾#”È	'Ây	Å)gž*´'ñ	PqšÂ3×*Š«ÂÐöbž3×*eŒá	g±ì©>#q Ž*ÃûÌÑ ú‘ê’AÑ %ú¨ ‘Ñ� AÁÿ Ä�4ÄêN„(W(*g	Ãþ	D[ ‚F„õ%‘•#î#/'X*$ßìób	¼~Ñ‘(Ñÿ Ä4Ä(|¶ç(q›èæd%c)Û£ÄŸ“%3Í¼„f&ö “xw%n	-WŸ3ÜÍ¼ T$N)*w*Œ*_Õ¶uÆ Ï'�Ài%Þ&HÖWl  :˜© …‹j &*š*j~½I
U
z
•z°ú%ëQ¨Ú"=0#ü*¤	 �°2'´çwN &(N)*w*Œ*›ó;*# ðLÚµd!Ê–—PíåT¦D¬WyE"Î&#')¦¬WE"#')M�ô_±rv¸\�ë@% ` 3%f �áßG÷ \¦�¬ëWÝ^W E"#')HW  :–P]íì\åT�DSëy†D*R*üS(m¸! 'wN)*w*Œ*Úœë	cj­ÿÑ
S§ã÷)ÅÜ!Ó'iu-Øµ$Ï) —ŸOÍü±Îtƒ4„r¡Ú8"Ó"y%+›wó� ÿ!N)*;*w*Œ*„Ž¼ É ö “	(´ù}xÅw–%\)n	 -�ÉS¶Wº4PÄëŠŸ3N…ÜöÍåò¼ )!P!T$²$Û$®%ü'N)**w*Œ*üŽš`Pè=Ý$/+{"(
³u!�#ô&÷%w)Øë …Ë$Š´£ ò ü �Ïó$%¸ßàìí‘Ößóô¦b	c	¡ OX²¼½A±,R�·ÕaSÌ%¸÷ «!Å)**Ç±-§„ÊÙ!l"«% â¾$iÅÏt%wa¯O¹ÊØ$�*˜*?rh›.�ÜñúÈÛ?
ß	Ö‹BŽšŠ»;"Âú…É_dHOÖ‹´ \"÷%Œ&w)-`(åG8s–(¦A·÷ P]íì\åT¦�DS¬ëWÝ^W y†E"Î&#')D*R*Î~ÃaÛ#Ë'4(@*Úd!ü Ïß¦A·%÷°#`J‘ �'…)5½øD+:  ?rFJwŠ¯�Ñ
Sàa¥ßþS(üá�2SƒÇÎ£§«¹ßãCGh›: W Ä !?!o#ö$ë()“)—)÷) *P*z*˜*¸¦²A�·*ÃÚÑ
Sª[d§ã!ˆ(÷)Š„–ÂdjŽ—¢C¬Œ#—#í#ù#ù&
'#Ü`‡&6 -&�±¸ô’î $$Ç&±EG&‚»¯ S!å!!%PíåTDy‹ ù´Î*üQ¨ÚŽš"=0`P#ü*è¤	 =�°Ý$2'/+u Ô Þžý£"t#”ù
ˆ¥h?58ÿM#~ÕÓ
ØÚ)qö›Ã¶Z­ÁÞºñ
x¦;Üqðö¬WÐû!¢›ÃE"-$#')Ü 11ÐgŽ´Ã6Ÿð8�›Á	ã	º
aÞ|½Vì½±ìíBU_n©W•Ó8k˜G ô!z"Ü">#‘#Û#Þ$Ë'4(R(Ô)@*0+œåÏf'TáÉ!�"f;ê?wa¹˜*LO	ÿ×†Y _"T#'Ó(•)ù*%nn—r—ŸOüƒ¡O	ÿ×Y •)ù*q›# ð(
³rLu!{"�#ô&¼_ª'wR�&�º&'³ î%e	‰Q'“i
„TÎd~f&
) Iw !&Ü �í•Ó–Âà	¢ø´ïzCfŒ#í#ù&ÌT—ekŒ˜)­B#˜#ú#Ê%'ò %àí’«ÂÖô€c	¡¡½Ð6,ö•åbÒS &!Ÿ!¬!Å)Ú*Ù_³.m¼vFìÈ'a(-+q åG8s…š	æËÂšË$–(–™ž¡þ©N	Ž
Äñ>¸7:zÖÛ…W¨ÄËþ[ ¬ ¾ a"¢"¥"s#»$)&Œ(a)ª)€ ­ ¼ É  )´úÛ®Å�\]ŒÜM�žS¶Ó>5Å3ëìùO…eöI©Öåò)²$%%ð%X(�)*×*¤ ��;ÔG›P±²îÕcÍ¸¹†">%�&’(µ(*Wög	ð
w—» ¡b&S'o(x(e*’*Ž¨=)œÒó#Ö$D'õ*¼ É  )´ÅÜMS>5Å3Iåò)%ð%×*™¡þN	ÄzÖÛ…¨ÄË¢"¥"s#)&Œ(–ž©Ž
ñ>¸7:Wþ[ ¬ ¾ a"»$a)ª)å #ÛyÍ¨Öm›ÔáÝë¬$(%G&`*¹ jofvD1K¾â*ve´óÿ/€ïI—Áôñ"æ)ü÷×Ø†–™ž¡nþ©ÿN	Ž
Äñ>¸7:z2þÖÛ …¨ÎW’¨ÄËþ_Š�[ ¬ ¾ Á 7!Ò!a"¢"¥"s#»$&)&ˆ&m'Œ(ù(a)ª)¤ ò ��%;Ôàí’«ÂÖô€c	G¡›¡P½Ð±²6,îö•ÕåbÒScÍ¸¹ &!Ÿ!¬!†">%�&’(µ(Å)*Ú*¶{¤4
Õ
^)(7šÞc!%7'ü(H)Ä ¾
©€²KÁã(%íQ&±(3)•*¡*‚u
”
Wuè@™–ž"«"_&w#¯?ïP.‡Ù C  #¶$,%W%'Ð)i;en½
QJ0YKâ!'¢½'°(¤)·$}Ö
©nœ`âPßt$)%H&© Ò Þ¹¿“™¬°Òû"·#]$K ë§ßÍ*’B®&tOy&)(ðÄ1•½ Ê êÆ^	næó*W¾âÏÃ^(sÉï…‹'"á½õ2|å ,!#;$>&v(™(i)*K © Ò Þü¹ë§sÖß¿Í*“’B™®Ÿ¬°&tÒûO"·#]$y&#()aM/ç�öÚˆ¹#N$·$&Ó*'rÈmà
ç�{6 £ �"ª"-&�q!³#‰Ž‹O²¡&Ï(¤*Ì 7]°Lþõ; wt Õ(ß()¼Ä$á{Ÿˆ	I øùÓpü’™Yo„f § ò ~"ƒ$é*Šeo÷íò³p!Š$‘%×)ä)[ è ~¹ßÌ'gºÐg‚ºÅ;')	V	 
è
"´Z<p?äËÖ?HeF„§áN{!É!*#°#7$õ% &x&‚(ÿ(Fn…
¨&—x‰Èêñ!'#ë%@Ò
	F$0Ç2þ$b'q)( åBÒX¦÷Ç	$1n“pYQ/p"¯"##>$‰%Ã%\&'œ(¿)Ó)5½SÚœëŒø‘5D+:@LO[	Qc’ý"•#±$~&	£	k®aKt ³àã’ ð#ã&1'ž'†)Z*Iü™Y~"Äáˆ	 ùp’o§ ƒ$é*ÒX¦÷$1npY‰%\&œ(Ó)¼${ŸøÓ„f ò Ø†nÿ2þ ¨Î’_Š�Á 7!Ò!&ˆ&m'ù(a'MrÈm/ç�à
çö�Úˆ{6 £ �"ª"¹#N$·$&-&Ó* Å¹¸Š�m‡3M)Yw£*d $ÿÂÔ	A
ÃC(£r$¡%Ç‡'cJ&>@sˆŠp >�giœq s 9!~#È$µ&(e(g(Î(Í 8Ù^‡o±'M)"_ƒ¡“¼ÿ&°!]`ŽöÕ< – 8!T!Ó!æ!_"T#Ç$"%&´&'n' (,)F)‚*Ç N²”ÿF2þH¸wˆ¨ÎŠðt ~ Á È Ý Í"]#á%ˆ&Ñ'®(Õ(ß(ù())””Ø&j
…UÏ4eÎ½g&)
+ ! QE�¥�éîˆ æìDB	P	U‘–·ÆÒ;ð E·…ØÞòà%<[Ìí5V.^Í{®ÞFJzº Z Ð ²!":";#D%¨%ç%Ž(¡(–)ú*?Artˆ‰œxËéØ=çôhj›�°1 r Ž ¯ ½! "d"##%e%¶&(f(Í(O)d)é)*<*x*�* Ý —£t‘"‚1
Ä|âî‚–ÔàHú"÷#F%á*^ Ì›T…—ÃekwŒj�2	j	á	þ	€˜Ô£ù”µ	<ð){DI­Ag‡B#�#˜# #î#ú#c$¸%Ê%ø%ú&'/'x)X*� ® I×ûÜXm®¡ž]	Œ`;ž¤·¶Óýk™®ìùªP…e†öŒª×k !$!6!è"²$%&X(‚)**›*¥ �;xÕ{Gb›Q²°ïÕPcºËÎ¹ñÚ!m"‡"E#Ù$û$>%¬%ž&“(µ(*�*™*¨*; ´§ÛÄ\óÖ	a
Ÿ
Ò
$àTb¦)B«†ß\e¸¢ÀƒFs¨äd*!Ü#Ç%Ñ%&C&Ì'5(‰(ø)A* ! � § ® Q^§EI�¥×û…ÜXm®Ýãýd�¡éîˆží ]fnæìùD		B	P	�
JUãøEŒ‘–·ÆÒ;`d�;ž¤­·ð E]·Þ |¶ÓNT…˜¯ØÞýRVòk¦Sÿà%<™Ÿ®ìù[ª± P…Ìí5Ve‘Ê.^r†Íö{Œ®¸¼ÞFJªÐ×z‡xºï  Z k ½ Ð !$!6!P!²!":"?"X"§"è"ó"#;#¦$²$Û$õ$ù$%D%ž%¨%ç%&X(Ž(¡(Â(ê(î(`)‚)–)**E*S*›*Ý*ú* ++; F } 7´§Û"—ÄØÚz–MT\‰ó…	Ð	Ö	ø	C
a
Š
Ÿ
Ã
Ò
÷
$,cØàKTZ\b‰™õ¦×3)ÚÜBI\«8~š½ÖÜ†}…ßîÀÂÑº?\e¸¢À~ƒFs¨Ûädo¦*‹¨éø!L!•!	"Æ"Ü#$$&$($x$›%Ç%Ñ%&%&2&C&…&¼&™'Ì'Ø'5(B(‰(ö())ø))*A*s*++è;d‹‘nµá~˜ùÎyÁÊ#j%ß&(Ù)4 µ˜ PÖýØ	]
�
Ð
Ô
§ÞÃã÷¨5„lÞG´¦`e'­åû´0€ÇXI†Ê¢ª]×Ý] F"n#½$%Ø%0&$'‹()¬)ö)ú)kÖ�?CE¾t	ê	J
V
q
{
8–	{æ±ÆÐûå&gìtÄÌÝ( $6%5&¾*Ñ i|©Ýóa‰ð >APWg6
Ù
G²_X¬ÌáSÔ S£RlÑú’¥Òœ ·!%%ó%€(„)Õ?Àd�á?ÏÖ"
ö%,^” þû<ûI%.1=I‘� »ÜñôúýérÈE½ýQ{ ~!Â"R$£$V(°*$ ña—AõÛ“.EvÄïMc& a Í#$$$I$4%[%È&<(ñ(¶)Ä*   ! c Qò@E�¥èPúÿ;E]d{ˆ” ªÄÕ=‹�éîˆ �æìDËB	P	/
­
UÛ‘–£·ÆÒï;xdð E·€ÆZ…žØÞ ò*Ç‚¨àé%<‘õ4[n”žµÄá2s~˜Ìíù+5V|Î.^y Í{Ÿ®´ÁÐÞ6<FJŒ+zº N Z Ð !²!à!""+":"Ã"&#;#Ê#õ#W$D%j%¨%ç%ß&((?((Ž(¡(–)Ù)†*ú*GRy…¿Îžr)°gspê+:»œý\o!'  i$     
ê  +@        +@XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`]Uí»EnyÒH'b½S„íçøàM Aö ô‚}o§ÑbáihUExQfÉPFôojlvø,NÑ"c€ö£„YîµjØXÎ	XÙˆaÔ‘ÿ©YãN�8àvØð>E|Ò‹«QTŠn«Vñá´÷¤šç�ã€B9�VÔfŽ=yÑU­Ò‚dŽ‘G„ïNµƒ¨ÃAÒS/’÷;ºÁê�¸AN f˜!Ø4�Š `EÔ[®Dfnú²èkRÝGÒI´×•øAA•]KMS`‡DEêACÔÑJ®Û®Ñ²"¢„âå“NEbÿà!°ßZw»›æøæ„ÑšëI¨êAm§¯Ù­�zÔE¡†*™¡¸WG¾:z¡íMù6îøëeÕ¨÷E¨£vtVžkì¾¹ƒ�x"/b:&Ÿ‹ç[AŽ`TJ��¾Ýb¹¸b^gø‚–ŠíŽ´£Øç˜ª»ZŠ»kÀâ¨º‡Êa‚ë,¸!zIêj•¨}7ªaY¨ä³ÕÐUÙøØ;ŒÔš¡ÛƒÚV�u-M-ëD šè­ÿÓïgPØí™í„Ñ¢Ea�j¡‘&Ó´æhøáK¯µ:¾"4êžté«Û>5 Qù¦5†xŒª:�‹èæ»x‚=U½;Òƒ´�‰àqã‹½âBm6š 2!+©½†8V´RÓzÕ¦ÛZ"bÑ—Å›Òh5jjà^ûTâíýD’g¸=F X‚ô½uƒmB:âþ(½µý§G4}Àþ�Sú$>.Å$Þ+m%zÿª’N=×NTOˆãA”É!ö-JãVsŽ¶„È›ASî»h7†"îÍÇ�4bŠ;KnúþQ8ïŠÁ}»jå@è5â8q�ØxãíÚë�­ÿHxãŽ»âîëüû�\6�KOcž #®?ëŽÿãöûßpÿý¶�¨ø?m˜ÐÖ¿ê	Ò©´DCÛ|�`ÐU¬H£°}‚6tôOãÙU]hºúÿ~s�Òm·D›¤D×c±�Eÿ(÷âûê®î§¾ÛH¾ÕtCLõ•ê~Ô9_4¶¯U˜	0c¨d7éº“F¬¼jÒkÀS–aR·âˆÚí?0¯†:è�Nîuˆ© tCÓ™x·^ÓÛw~…òÔúzáÛ�zôú‹�ê XVÒj…6¾·EyŠ´”ÞíÀ¶CuPGhýš˜f´x5Ôâ‡¸©Fèõ×�Dˆ÷ê[çF¢éX`Ëî‹Îãt=½Ô¨u+]…›H"«ÛÕ€àRÛmgDjúÁêöê�Þ+ã¸Xò(ª—Ýà!‰�5Š44a…zxDU?ªÛêÅÚ×5á¦WæMy¡ˆWÛ¨~#twÕ)º8¶„[PÓ`cxY�Ý"4;˜RCL¸4A„QAD]Q†Q†g†‘…äw‡ÒŽ4^a×XE�A�aë»û†NM_®D Q…á�A†ø wGNÁ×¸jvÛ¦îÄÀmkÏÿþ\§ƒÅ<èPðˆÊ‹™Šj½öhòêƒX„Á_ƒøºèq6¾@.¯û³Õ�Bâ…‹!AM+î:èQÕ×.$Çú�0_ÿ~t#î0õSÜ‰”g~5C×ý#Ü¢�’ˆÕƒ»íLš×Pý'ý¢`†à°�Øêv"¤±
UW.CÿÅ÷ r
¯¨UwgØ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IS1îD±øÐØÔëBO\¥×Â~ä^däØÍZ•3†leYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾’¶˜3EDàZ!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇÔØÔƒ?]/ü=À>=ösÅu3¶ñH1?¯öÂ°Æd3Ó_7âð†<=Æ2ðÉÆ’õ¨¥GA›H/C¹*‡µÖmôáO™q=‘•eQªjK½P       +?  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Y#<?xml version="1.0"?><Features recycledIDs="54,50,49,48,45,37,38" nextValidID="59"><Feature recentID="58" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-6002" color="#ffffff" type="standard"/><Q name="strain"><V text="C57BL/6"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="map"><V text="9 57.98 cM"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="chromosome"><V text="9"/></Q></Feature><Feature recentID="57" name="Dcaf1" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-6002" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="db_xref"><V text="321006" predef="GeneID"/><V text="MGI:2445220" predef="MGI"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="DDB1 and CUL4 associated factor 1"/></Q></Feature><Feature recentID="56" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1482-1591" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="55" name="Dcaf1" directionality="1" translationMW="168931.56" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1482-6002" color="#993366" type="standard" translated="1"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="product"><V text="DDB1- and CUL4-associated factor 1 isoform 1"/></Q><Q name="EC_number"><V text="2.7.11.1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="protein_id"><V text="NP_001360859.1"/></Q><Q name="db_xref"><V text="CCDS90657.1" predef="CCDS"/><V text="321006" predef="GeneID"/><V text="MGI:2445220" predef="MGI"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="isoform 1 is encoded by transcript variant 1;&#10;protein VPRBP; serine/threonine-protein kinase VPRBP; Vpr (HIV-1) binding protein"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="53" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2085-2087" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="52" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2202-2306" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="51" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2241-2243" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="47" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4161-4163" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="46" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4170-4172" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="44" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4410-4478" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="43" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4413-4415" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="42" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4476-4478" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="41" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4749-5348" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: WD repeat-like region"/></Q></Feature><Feature recentID="40" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5202-5225" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: DWD box 1"/></Q></Feature><Feature recentID="39" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5310-5333" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: DWD box 2"/></Q></Feature><Feature recentID="36" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5730-5999" color="#a6acb3" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Interaction with NF2. /evidence=ECO:0000250"/></Q></Feature><Feature recentID="35" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1592-1668" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="34" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1669-1742" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="33" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1743-1856" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="32" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1857-1994" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="31" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1995-2504" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="30" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2505-2606" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="29" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2607-2765" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="28" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2945" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="27" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2946-3155" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="26" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3156-3325" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="25" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3326-3450" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="24" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3451-4714" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="23" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4715-4913" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="22" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4914-4996" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="21" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4997-5081" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="20" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5082-5315" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="19" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5316-5409" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="18" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5410-5514" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="17" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5515-5588" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="16" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5589-5690" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="15" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5691-5943" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="14" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5944-6002" color="#708090" type="standard"/><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="13" name="Myc" directionality="1" translationMW="1203.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6002" maxFusedRunOn="6002" detectionMode="exactProteinMatch"><Segment range="1452-1481" color="#cc99b2" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Myc (human c-Myc oncogene) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="12" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10141-10245" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10071" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9280-10071" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10072-10140" color="#ccffcc" type="standard" translated="1"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8167-8173" color="#ffffff" type="standard"/><Segment range="8174-8191" color="#ffffff" type="standard"/><Segment name="-35" range="8192-8197" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8212-8233" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8143-8159" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8521-9109" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6586-6588" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6589-6591" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6592-7305" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="7305" maxFusedRunOn="7305" detectionMode="exactProteinMatch"><Segment range="6586-6588" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="6589-6591" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6592-7302" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7467-7981" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1451" color="#a6acb3" type="standard"/><Q name="regulatory_class"><V predef="other"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   Ì<?xml version="1.0"?><Primers recycledIDs="12,8,15,13,11,2,10,7,3,4,6,5,0,1,9,14" nextValidID="16"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>f3f452b3-3b55-4cf2-95d8-a7da8a8ff5e5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="6:35:42">2026.2.2</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17." authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA"/>
</References>
</Notes>
  ž™     
ë  +A        +AXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`]Uí»EnyÒH'b½S„íçøàM Aö ô‚}o§ÑbáihUExQfÉPFôojlvø,NÑ"c€ö£„YîµjØXÎ	XÙˆaÔ‘ÿ©YãN�8àvØð>E|Ò‹«QTŠn«Vñá´÷¤šç�ã€B9�VÔfŽ=yÑU­Ò‚dŽ‘G„ïNµƒ¨ÃAÒS/’÷;ºÁê�¸AN f˜!Ø4�Š `EÔ[®Dfnú²èkRÝGÒI´×•øAA•]KMS`‡DEêACÔÑJ®Û®Ñ²"¢„âå“NEbÿà!°ßZw»›æøæ„ÑšëI¨êAm§¯Ù­�zÔE¡†*™¡¸WG¾:z¡íMù6îøëeÕ¨÷E¨£vtVžkì¾¹ƒ�x"/b:&Ÿ‹ç[AŽ`TJ��¾Ýb¹¸b^gø‚–ŠíŽ´£Øç˜ª»ZŠ»kÀâ¨º‡Êa‚ë,¸!zIêj•¨}7ªaY¨ä³ÕÐUÙøØ;ŒÔš¡ÛƒÚV�u-M-ëD šè­ÿÓïgPØí™í„Ñ¢Ea�j¡‘&Ó´æhøáK¯µ:¾"4êžté«Û>5 Qù¦5†xŒª:�‹èæ»x‚=U½;Òƒ´�‰àqã‹½âBm6š 2!+©½†8V´RÓzÕ¦ÛZ"bÑ—Å›Òh5jjà^ûTâíýD’g¸=F X‚ô½uƒmB:âþ(½µý§G4}Àþ�Sú$>.Å$Þ+m%zÿª’N=×NTOˆãA”É!ö-JãVsŽ¶„È›ASî»h7†"îÍÇ�4bŠ;KnúþQ8ïŠÁ}»jå@è5â8q�ØxãíÚë�­ÿHxãŽ»âîëüû�\6�KOcž #®?ëŽÿãöûßpÿý¶�¨ø?m˜ÐÖ¿ê	Ò©´DCÛ|�`ÐU¬H£°}‚6tôOãÙU]hºúÿ~s�Òm·D›¤D×c±�Eÿ(÷âûê®î§¾ÛH¾ÕtCLõ•ê~Ô9_4¶¯U˜	0c¨d7éº“F¬¼jÒkÀS–aR·âˆÚí?0¯†:è�Nîuˆ© tCÓ™x·^ÓÛw~…òÔúzáÛ�zôú‹�ê XVÒj…6¾·EyŠ´”ÞíÀ¶CuPGhýš˜f´x5Ôâ‡¸©Fèõ×�Dˆ÷ê[çF¢éX`Ëî‹Îãt=½Ô¨u+]…›H"«ÛÕ€àRÛmgDjúÁêöê�Þ+ã¸Xò(ª—Ýà!‰�5Š44a…zxDU?ªÛêÅÚ×5á¦WæMy¡ˆWÛ¨~#twÕ)º8¶„[PÓ`cxY�Ý"4;˜RCL¸4A„QAD]Q†Q†g†‘…äw‡ÒŽ4^a×XE�A�aë»û†NM_®D Q…á�A†ø wGNÁ×¸jvÛ¦î±0Zóÿÿ—)àñO:”<"2¢æbš¯}š<º Ö!ðWàþ.ºM¯�«þìõAcP¸¡bÈPSJû�NºuuË‰1þ£CLÿßƒûŒ=T÷"eß�PÃuÿH÷(„ d¢Dµ`îûS&µÔ…?E	ÿh˜á¸ì#v:�ˆ©,B•UË�ÿñ}À‚«ê]ÆYöM@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'RTÃÌ{‘,~465:Ð“Ã—)uðŸ¹™963V¥Lá›VU¦¤»ÑU …Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…d­¦LÑQ8ˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃ5‚65`Ï×Kÿp�}ŸÜñ]Lí„üROÆëý°†,1™ô×Íøƒ<!�qŒ¼2D1¤½j)QÐfÒÐîJ¡íu›}8SæDœOdeYTjš’ïET        +@   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  hå<?xml version="1.0"?><Features nextValidID="61"><Feature recentID="60" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1451" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="59" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6003-6003" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="58" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6003-6003" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="57" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="56" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="55" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7468-7982" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="54" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="53" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="7306" maxFusedRunOn="7306" detectionMode="exactProteinMatch"><Segment range="6587-6589" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="6590-6592" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6593-7303" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="52" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6587-6589" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6590-6592" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6593-7306" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="51" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8522-9110" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="50" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8144-8160" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="49" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8213-8234" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="48" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8168-8174" color="#ffffff" type="standard"/><Segment range="8175-8192" color="#ffffff" type="standard"/><Segment name="-35" range="8193-8198" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="47" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10072" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9281-10072" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10073-10141" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="46" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10142-10246" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="45" name="Myc" directionality="1" translationMW="1203.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6002" maxFusedRunOn="6002" detectionMode="exactProteinMatch"><Segment range="1452-1481" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Myc (human c-Myc oncogene) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="44" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5944-6002" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="43" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5691-5943" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="42" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5589-5690" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="41" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5515-5588" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="40" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5410-5514" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="39" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5316-5409" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="38" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5082-5315" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="37" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4997-5081" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="36" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4914-4996" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="35" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4715-4913" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="34" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3451-4714" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="33" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3326-3450" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="32" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3156-3325" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="31" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2946-3155" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="30" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2945" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="29" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2607-2765" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="28" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2505-2606" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="27" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1995-2504" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="26" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1857-1994" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="25" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1743-1856" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="24" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1669-1742" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="23" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1592-1668" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="22" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5730-5999" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Interaction with NF2. /evidence=ECO:0000250"/></Q></Feature><Feature recentID="21" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5655-5999" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="20" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-5462" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="19" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5310-5333" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: DWD box 2"/></Q></Feature><Feature recentID="18" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5202-5225" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: DWD box 1"/></Q></Feature><Feature recentID="17" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4749-5348" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: WD repeat-like region"/></Q></Feature><Feature recentID="16" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4476-4478" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="15" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4413-4415" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="14" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4410-4478" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="13" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4227-4319" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="12" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4170-4172" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="11" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4161-4163" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="10" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4140-4142" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphothreonine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="9" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3960-3962" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="8" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3579-3581" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="N6-acetyllysine.&#10;/evidence=ECO:0007744|PubMed:23806337; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); acetylation site"/></Q></Feature><Feature recentID="7" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2241-2243" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="6" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2202-2306" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="5" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2085-2087" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="4" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1902-2978" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Protein kinase-like. /evidence=ECO:0000250"/></Q></Feature><Feature recentID="3" name="Dcaf1" directionality="1" translationMW="168931.56" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1482-6002" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="2.7.11.1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS90657.1" predef="CCDS"/><V text="321006" predef="GeneID"/><V text="MGI:2445220" predef="MGI"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="isoform 1 is encoded by transcript variant 1;&#10;protein VPRBP; serine/threonine-protein kinase VPRBP; Vpr (HIV-1) binding protein"/></Q><Q name="product"><V text="DDB1- and CUL4-associated factor 1 isoform 1"/></Q><Q name="protein_id"><V text="NP_001360859.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1482-1591" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="Dcaf1" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-6002" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="321006" predef="GeneID"/><V text="MGI:2445220" predef="MGI"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="DDB1 and CUL4 associated factor 1"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-6002" color="#ffffff" type="standard"/><Q name="chromosome"><V text="9"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="9 57.98 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature></Features>
  &J<?xml version="1.0"?><Primers nextValidID="16"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="DCAF1-R" sequence="cccgctcgagctcactcattcagagataagatgatgtcatcttcc" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2026-01-26T03:36:11Z"><BindingSite location="5968-6012" boundStrand="1" annealedBases="cccgctcgagctcactcattcagagataagatgatgtcatcttcc" meltingTemperature="69"><Component hybridizedRange="5968-6012" bases="cccgctcgagctcactcattcagagataagatgatgtcatcttcc"/></BindingSite><BindingSite simplified="1" location="5968-6012" boundStrand="1" annealedBases="cccgctcgagctcactcattcagagataagatgatgtcatcttcc" meltingTemperature="69"><Component hybridizedRange="5968-6012" bases="cccgctcgagctcactcattcagagataagatgatgtcatcttcc"/></BindingSite></Primer><Primer recentID="1" name="DCAF1-F" sequence="cggatcccaattggccaccatggaacaaaaactcatctcagaagaggatctgatgactacagtagtggtacatgtggactcca" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2026-01-26T03:35:21Z"><BindingSite location="1429-1511" boundStrand="0" annealedBases="cggatcccaattggccaccatggaacaaaaactcatctcagaagaggatctgatgactacagtagtggtacatgtggactcca" meltingTemperature="74"><Component hybridizedRange="1429-1511" bases="cggatcccaattggccaccatggaacaaaaactcatctcagaagaggatctgatgactacagtagtggtacatgtggactcca"/></BindingSite><BindingSite simplified="1" location="1429-1511" boundStrand="0" annealedBases="cggatcccaattggccaccatggaacaaaaactcatctcagaagaggatctgatgactacagtagtggtacatgtggactcca" meltingTemperature="74"><Component hybridizedRange="1429-1511" bases="cggatcccaattggccaccatggaacaaaaactcatctcagaagaggatctgatgactacagtagtggtacatgtggactcca"/></BindingSite></Primer><Primer recentID="2" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="9903-9922" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="9903-9922" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="9903-9922" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="9903-9922" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="3" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="7239-7260" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="7239-7260" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="7239-7260" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="7239-7260" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="4" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="6892-6911" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="6892-6911" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="6892-6911" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="6892-6911" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="5" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="6178-6195" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="6178-6195" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="6178-6195" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="6178-6195" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="6" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="6405-6424" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="6405-6424" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="6405-6424" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="6405-6424" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="7" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="8350-8367" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="8350-8367" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="8350-8367" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="8350-8367" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="8" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="10704-10726" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="10704-10726" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="10704-10726" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="10704-10726" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="9" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="10" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="8601-8620" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="8601-8620" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="8601-8620" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="8601-8620" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="11" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="10313-10331" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="10313-10331" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="10313-10331" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="10313-10331" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="12" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="10854-10873" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="10854-10873" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="10854-10873" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="10854-10873" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="13" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="10369-10391" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="10369-10391" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="10369-10391" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="10369-10391" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="14" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="15" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="3173-3184" boundStrand="0" annealedBases="GAGCAGATTGTA" meltingTemperature="37"><Component bases="CGGCATCA"/><Component hybridizedRange="3173-3184" bases="GAGCAGATTGTA"/></BindingSite><BindingSite location="10491-10510" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="10491-10510" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="3173-3184" boundStrand="0" annealedBases="GAGCAGATTGTA" meltingTemperature="37"><Component bases="CGGCATCA"/><Component hybridizedRange="3173-3184" bases="GAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="10491-10510" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="10491-10510" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>f3f452b3-3b55-4cf2-95d8-a7da8a8ff5e5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="10:56:50">2026.1.29</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17." authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" title="Generation of T-cell receptor retrogenic mice."/>
</References>
</Notes>
  ¢ì     
ë  +D        +DXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`]Uí»EnyÒH'b½S„íçøàM Aö ô‚}o§ÑbáihUExQfÉPFôojlvø,NÑ"c€ö£„YîµjØXÎ	XÙˆaÔ‘ÿ©YãN�8àvØð>E|Ò‹«QTŠn«Vñá´÷¤šç�ã€B9�VÔfŽ=yÑU­Ò‚dŽ‘G„ïNµƒ¨ÃAÒS/’÷;ºÁê�¸AN f˜!Ø4�Š `EÔ[®Dfnú²èkRÝGÒI´×•øAA•]KMS`‡DEêACÔÑJ®Û®Ñ²"¢„âå“NEbÿà!°ßZw»›æøæ„ÑšëI¨êAm§¯Ù­�zÔE¡†*™¡¸WG¾:z¡íMù6îøëeÕ¨÷E¨£vtVžkì¾¹ƒ�x"/b:&Ÿ‹ç[AŽ`TJ��¾Ýb¹¸b^gø‚–ŠíŽ´£Øç˜ª»ZŠ»kÀâ¨º‡Êa‚ë,¸!zIêj•¨}7ªaY¨ä³ÕÐUÙøØ;ŒÔš¡ÛƒÚV�u-M-ëD šè­ÿÓïgPØí™í„Ñ¢Ea�j¡‘&Ó´æhøáK¯µ:¾"4êžté«Û>5 Qù¦5†xŒª:�‹èæ»x‚=U½;Òƒ´�‰àqã‹½âBm6š 2!+©½†8V´RÓzÕ¦ÛZ"bÑ—Å›Òh5jjà^ûTâíýD’g¸=F X‚ô½uƒmB:âþ(½µý§G4}Àþ�Sú$>.Å$Þ+m%zÿª’N=×NTOˆãA”É!ö-JãVsŽ¶„È›ASî»h7†"îÍÇ�4bŠ;KnúþQ8ïŠÁ}»jå@è5â8q�ØxãíÚë�­ÿHxãŽ»âîëüû�\6�KOcž #®?ëŽÿãöûßpÿý¶�¨ø?m˜ÐÖ¿ê	Ò©´DCÛ|�`ÐU¬H£°}‚6tôOãÙU]hºúÿ~s�Òm·D›¤D×c±�Eÿ(÷âûê®î§¾ÛH¾ÕtCLõ•ê~Ô9_4¶¯U˜	0c¨d7éº“F¬¼jÒkÀS–aR·âˆÚí?0¯†:è�Nîuˆ© tCÓ™x·^ÓÛw~…òÔúzáÛ�zôú‹�ê XVÒj…6¾·EyŠ´”ÞíÀ¶CuPGhýš˜f´x5Ôâ‡¸©Fèõ×�Dˆ÷ê[çF¢éX`Ëî‹Îãt=½Ô¨u+]…›H"«ÛÕ€àRÛmgDjúÁêöê�Þ+ã¸Xò(ª—Ýà!‰�5Š44a…zxDU?ªÛêÅÚ×5á¦WæMy¡ˆWÛ¨~#twÕ)º8¶„[PÓ`cxY�Ý"4;˜RCL¸4A„QAD]Q†Q†g†‘…äw‡ÒŽ4^a×XE�A�aë»û†NM_®D Q…á�A†ø wGNÁ×¸jvÛ¦îNÄÀmkÏÿþ\§ƒÅ<èPðˆÊ‹™Šj½öhòêƒX„Á_ƒøºèq6¾@.¯û³Õ�Bâ…‹!AM+î:èQÕ×.$Çú�0_ÿ~t#î0õSÜ‰”g~5C×ý#Ü¢�’ˆÕƒ»íLš×Pý'ý¢`†à°�Øêv"¤±
UW.CÿÅ÷ r
¯¨UwgØ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IS1îD±øÐØÔëBO\¥×Â~ä^däØÍZ•3†leYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾’¶˜3EDàZ!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇÔØÔƒ?]/ü=À>=ösÅu3¶ñH1?¯öÂ°Æd3Ó_7âð†<=Æ2ðÉÆ’õ¨¥GA›H/C¹*‡µÖmôáO™q=‘•eQªjK½P       +C   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  o²<?xml version="1.0"?><Features recycledIDs="0" nextValidID="65"><Feature recentID="64" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-6002" color="#ffffff" type="standard"/><Q name="chromosome"><V text="9"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="9 57.98 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="63" name="Dcaf1" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-6002" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="321006" predef="GeneID"/><V text="MGI:2445220" predef="MGI"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="DDB1 and CUL4 associated factor 1"/></Q></Feature><Feature recentID="62" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1482-1591" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="61" name="Dcaf1" directionality="1" translationMW="168931.56" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1482-6002" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="2.7.11.1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS90657.1" predef="CCDS"/><V text="321006" predef="GeneID"/><V text="MGI:2445220" predef="MGI"/></Q><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="isoform 1 is encoded by transcript variant 1;&#10;protein VPRBP; serine/threonine-protein kinase VPRBP; Vpr (HIV-1) binding protein"/></Q><Q name="product"><V text="DDB1- and CUL4-associated factor 1 isoform 1"/></Q><Q name="protein_id"><V text="NP_001360859.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="60" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1902-2978" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Protein kinase-like. /evidence=ECO:0000250"/></Q></Feature><Feature recentID="59" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2085-2087" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="58" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2202-2306" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="57" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2241-2243" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="56" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3579-3581" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="N6-acetyllysine.&#10;/evidence=ECO:0007744|PubMed:23806337; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); acetylation site"/></Q></Feature><Feature recentID="55" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3960-3962" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="54" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4140-4142" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphothreonine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="53" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4161-4163" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="52" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4170-4172" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="51" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4227-4319" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="50" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4410-4478" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="49" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4413-4415" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="48" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4476-4478" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="47" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4749-5348" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: WD repeat-like region"/></Q></Feature><Feature recentID="46" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5202-5225" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: DWD box 1"/></Q></Feature><Feature recentID="45" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5310-5333" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: DWD box 2"/></Q></Feature><Feature recentID="44" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5460-5462" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0000250|UniProtKB:Q9Y4B6; propagated from UniProtKB/Swiss-Prot (Q80TR8.4); phosphorylation site"/></Q></Feature><Feature recentID="43" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5655-5999" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"/></Q></Feature><Feature recentID="42" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5730-5999" color="#a6acb3" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q80TR8.4);&#10;Region: Interaction with NF2. /evidence=ECO:0000250"/></Q></Feature><Feature recentID="41" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1592-1668" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="40" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1669-1742" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="39" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1743-1856" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="38" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1857-1994" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="37" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1995-2504" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="36" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2505-2606" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="35" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2607-2765" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="34" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2766-2945" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="33" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2946-3155" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="32" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3156-3325" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="15" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3326-3450" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="12" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3451-4714" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="10" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4715-4913" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="9" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4914-4996" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="8" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4997-5081" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="7" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5082-5315" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="6" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5316-5409" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="5" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5410-5514" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="4" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5515-5588" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="3" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5589-5690" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5691-5943" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5944-6002" color="#708090" type="standard"/><Q name="gene"><V text="Dcaf1"/></Q><Q name="gene_synonym"><V text="B930007L02Rik; mKIAA0800; Vprbp"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="11" name="RHEB" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="186" maxRunOn="6006" maxFusedRunOn="6006"><Segment range="6003-6003" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="3.6.5.2"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS5927.1" predef="CCDS"/><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog enriched in brain 2"/></Q><Q name="product"><V text="GTP-binding protein Rheb"/></Q><Q name="protein_id"><V text="NP_005605.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="Myc" directionality="1" translationMW="1203.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6002" maxFusedRunOn="6002" detectionMode="exactProteinMatch"><Segment range="1452-1481" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Myc (human c-Myc oncogene) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="31" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10145-10249" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10075" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9284-10075" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10076-10144" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8171-8177" color="#ffffff" type="standard"/><Segment range="8178-8195" color="#ffffff" type="standard"/><Segment name="-35" range="8196-8201" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8216-8237" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8147-8163" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8525-9113" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6590-6592" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6593-6595" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6596-7309" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="7309" maxFusedRunOn="7309" detectionMode="exactProteinMatch"><Segment range="6590-6592" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="6593-6595" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6596-7306" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7471-7985" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6004-6006" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="18" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6004-6006" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="16" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1451" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="14" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6003-6003" color="#ffffff" type="standard"/><Q name="chromosome"><V text="7"/></Q><Q name="db_xref"><V text="9606" predef="taxon"/></Q><Q name="map"><V text="7q36.1"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Homo sapiens"/></Q></Feature><Feature recentID="13" name="RHEB" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="6003-6003" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog, mTORC1 binding"/></Q></Feature></Features>
  #Ñ<?xml version="1.0"?><Primers recycledIDs="0" nextValidID="16"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="9906-9925" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="9906-9925" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="9906-9925" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="9906-9925" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="2" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="7242-7263" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="7242-7263" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="7242-7263" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="7242-7263" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="3" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="1665-1676" boundStrand="1" annealedBases="CAGCTCGACCAG" meltingTemperature="47"><Component bases="CGTCGCCGTC"/><Component hybridizedRange="1665-1676" bases="CAGCTCGACCAG"/></BindingSite><BindingSite location="6634-6655" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="6634-6655" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="1665-1676" boundStrand="1" annealedBases="CAGCTCGACCAG" meltingTemperature="47"><Component bases="CGTCGCCGTC"/><Component hybridizedRange="1665-1676" bases="CAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="6634-6655" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="6634-6655" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="4" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="6895-6914" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="6895-6914" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="6895-6914" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="6895-6914" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="5" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="6181-6198" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="6181-6198" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="6181-6198" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="6181-6198" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="6" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="6408-6427" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="6408-6427" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="6408-6427" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="6408-6427" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="7" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="8353-8370" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="8353-8370" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="8353-8370" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="8353-8370" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="8" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="10707-10729" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="10707-10729" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="10707-10729" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="10707-10729" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="9" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="10" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="8604-8623" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="8604-8623" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="8604-8623" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="8604-8623" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="11" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="10316-10334" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="10316-10334" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="10316-10334" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="10316-10334" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="12" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="10857-10876" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="10857-10876" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="10857-10876" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="10857-10876" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="13" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="10372-10394" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="10372-10394" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="10372-10394" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="10372-10394" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="14" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="15" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="3173-3184" boundStrand="0" annealedBases="GAGCAGATTGTA" meltingTemperature="37"><Component bases="CGGCATCA"/><Component hybridizedRange="3173-3184" bases="GAGCAGATTGTA"/></BindingSite><BindingSite location="10494-10513" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="10494-10513" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="3173-3184" boundStrand="0" annealedBases="GAGCAGATTGTA" meltingTemperature="37"><Component bases="CGGCATCA"/><Component hybridizedRange="3173-3184" bases="GAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="10494-10513" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="10494-10513" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="3:32:33">2026.1.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  Tx   
  �  ›        ›XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`]Uí»En;µ¯?ÿùržó YCÂ#*.f)ª÷Ù£Ëªb?~âë¡ÄÚù º¿îÏT5Š,…4¯¸ë¡GW\¸“ê44Áýø1Ð�¸ÃÕOr&Q�øÕ7_ô�rˆJJ TKVïµ2k]@8PcôPŸö‰€n€>Â7c©ØŠ’Ä)U\¹ÿÜÈ*¾¡UÜeŸ`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%L<Ç¹ÇãCcS­	<9r—_	û‘y““c5jTÎ±•eQªjK½P XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'ò&õ•þèÒ�¼z »8¯ .û„†‡ŸËL º¶]*èR�]®$ÅšR[áíi=â¨[ÝžõžøVJÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@       š   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ).<?xml version="1.0"?><Features recycledIDs="1,2,3,4,5,6,7,8,9,10,12,15" nextValidID="32"><Feature recentID="11" name="RHEB" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="186" maxRunOn="1485" maxFusedRunOn="1485"><Segment range="1482-1482" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="3.6.5.2"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS5927.1" predef="CCDS"/><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog enriched in brain 2"/></Q><Q name="product"><V text="GTP-binding protein Rheb"/></Q><Q name="protein_id"><V text="NP_005605.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="Myc" directionality="1" translationMW="1203.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1517" maxFusedRunOn="1517" detectionMode="exactProteinMatch"><Segment range="1452-1481" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Myc (human c-Myc oncogene) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="31" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5624-5728" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5554" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4763-5554" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5555-5623" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3650-3656" color="#ffffff" type="standard"/><Segment range="3657-3674" color="#ffffff" type="standard"/><Segment name="-35" range="3675-3680" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3695-3716" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3626-3642" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4004-4592" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2069-2071" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2072-2074" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2075-2788" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="2788" maxFusedRunOn="2788" detectionMode="exactProteinMatch"><Segment range="2069-2071" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2072-2074" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2075-2785" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2950-3464" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1483-1485" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="18" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1483-1485" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="16" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1451" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="14" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-1482" color="#ffffff" type="standard"/><Q name="chromosome"><V text="7"/></Q><Q name="db_xref"><V text="9606" predef="taxon"/></Q><Q name="map"><V text="7q36.1"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Homo sapiens"/></Q></Feature><Feature recentID="13" name="RHEB" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-1482" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog, mTORC1 binding"/></Q></Feature><Feature recentID="0" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1482-1482" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature></Features>
   K<?xml version="1.0"?><Primers recycledIDs="0" nextValidID="16"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="1" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5385-5404" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5385-5404" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5385-5404" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5385-5404" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="2" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="2721-2742" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="2721-2742" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="2721-2742" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="2721-2742" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="3" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2113-2134" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2113-2134" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2113-2134" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2113-2134" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="4" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2374-2393" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2374-2393" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2374-2393" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2374-2393" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="5" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="1660-1677" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="1660-1677" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="1660-1677" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="1660-1677" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="6" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="1887-1906" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="1887-1906" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="1887-1906" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="1887-1906" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="7" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="3832-3849" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3832-3849" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="3832-3849" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3832-3849" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="8" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6186-6208" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6186-6208" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6186-6208" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6186-6208" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="9" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="10" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4083-4102" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4083-4102" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4083-4102" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4083-4102" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="11" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="5795-5813" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="5795-5813" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="5795-5813" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="5795-5813" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="12" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6336-6355" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6336-6355" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6336-6355" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6336-6355" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="13" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="5851-5873" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="5851-5873" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="5851-5873" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="5851-5873" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="14" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="15" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="5973-5992" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="5973-5992" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="5973-5992" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="5973-5992" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="3:31:39">2026.1.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  gý   	    Å        ÅXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`]Uí»EnóKÔ¿1¾Ÿˆ[í¡Æµ¨¡CÖ¢¾q½}‘Wz§T¡·IXE›nµè‘Ý<Fk›«ížöFi`æk¢&¸§mmTª†•)½ƒVŠ`€'V~iŠ
e�˜JaES¨4[ XÓª [®9QVÑãˆª±A–¨ÑU`p:ÝCRëS°†!“±0Zóÿÿ—)àñO:”<"2¢æbš¯}š<º Ö!ðWàþ.ºM¯�«þìõAcP¸¡bÈPSJû�NºuuË‰1þ£CLÿßƒûŒ=T÷"eß�PÃuÿH÷(„ d¢Dµ`îûS&µÔ…?E	ÿh˜á¸ì#v:�ˆ©,B•UË�ÿñ}À‚«ê]ÆYöM@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'RTÃÌ{‘,~465:Ð“Ã—)uðŸ¹™963V¥Lá›VU¦¤»ÑU …Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…d­¦LÑQ8ˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃ5‚65`Ï×Kÿp�}ŸÜñ]Lí„üROÆëý°†,1™ô×Íøƒ<!�qŒ¼2D1¤½j)QÐfÒÐîJ¡íu›}8SæDœOdeYTjš’ïET        Ä   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  9k<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="11" name="RHEB" directionality="1" translationMW="20481.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1482-2036" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="3.6.5.2"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS5927.1" predef="CCDS"/><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog enriched in brain 2"/></Q><Q name="product"><V text="GTP-binding protein Rheb"/></Q><Q name="protein_id"><V text="NP_005605.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="Myc" directionality="1" translationMW="1203.31" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2036" maxFusedRunOn="2036" detectionMode="exactProteinMatch"><Segment range="1452-1481" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Myc (human c-Myc oncogene) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="31" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6178-6282" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6108" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5317-6108" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6109-6177" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4204-4210" color="#ffffff" type="standard"/><Segment range="4211-4228" color="#ffffff" type="standard"/><Segment name="-35" range="4229-4234" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4249-4270" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4180-4196" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4558-5146" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2623-2625" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2626-2628" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2629-3342" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="3342" maxFusedRunOn="3342" detectionMode="exactProteinMatch"><Segment range="2623-2625" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2626-2628" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2629-3339" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3504-4018" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2037-2039" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="18" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2037-2039" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="16" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1451" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="15" name="mut" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2022-2022" color="#a6acb3" type="standard"/></Feature><Feature recentID="14" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-2036" color="#ffffff" type="standard"/><Q name="chromosome"><V text="7"/></Q><Q name="db_xref"><V text="9606" predef="taxon"/></Q><Q name="map"><V text="7q36.1"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Homo sapiens"/></Q></Feature><Feature recentID="13" name="RHEB" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1482-2036" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog, mTORC1 binding"/></Q></Feature><Feature recentID="12" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1482-1533" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="10" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1584-1610" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q15382.1);&#10;Region: Effector region. /evidence=ECO:0000305"/></Q></Feature><Feature recentID="9" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1584-1586" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Important for autoinhibition of GTPase activity. /evidence=ECO:0000269|PubMed:22819219; propagated from UniProtKB/Swiss-Prot (Q15382.1); other site"/></Q></Feature><Feature recentID="8" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1869-1871" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Phosphoserine, by MAPKAPK5. /evidence=ECO:0000250|UniProtKB:Q921J2; propagated from UniProtKB/Swiss-Prot (Q15382.1); phosphorylation site"/></Q></Feature><Feature recentID="7" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2023-2024" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Cysteine methyl ester. /evidence=ECO:0000269|PubMed:12906785, ECO:0000269|PubMed:15308774, ECO:0000269|PubMed:22002721, ECO:0007744|PDB:3T5G; propagated from UniProtKB/Swiss-Prot (Q15382.1); methylation site"/></Q></Feature><Feature recentID="6" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1534-1605" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="5" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1606-1673" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="4" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1674-1756" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="3" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1757-1813" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1814-1861" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1862-1943" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="0" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1944-2036" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature></Features>
  #<?xml version="1.0"?><Primers nextValidID="16"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="R" sequence="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-09-25T02:36:27Z"><BindingSite location="1998-2046" boundStrand="1" annealedBases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" meltingTemperature="73"><Component hybridizedRange="1998-2046" bases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg"/></BindingSite><BindingSite simplified="1" location="1998-2046" boundStrand="1" annealedBases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" meltingTemperature="73"><Component hybridizedRange="1998-2046" bases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg"/></BindingSite></Primer><Primer recentID="1" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5939-5958" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5939-5958" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5939-5958" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5939-5958" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="2" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="3275-3296" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3275-3296" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="3275-3296" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3275-3296" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="3" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2667-2688" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2667-2688" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2667-2688" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2667-2688" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="4" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2928-2947" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2928-2947" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2928-2947" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2928-2947" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="5" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="2214-2231" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2214-2231" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="2214-2231" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2214-2231" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="6" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="2441-2460" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2441-2460" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="2441-2460" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2441-2460" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="7" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="4386-4403" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4386-4403" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="4386-4403" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4386-4403" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="8" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6740-6762" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6740-6762" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6740-6762" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6740-6762" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="9" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="10" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4637-4656" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4637-4656" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4637-4656" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4637-4656" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="11" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="6349-6367" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6349-6367" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="6349-6367" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6349-6367" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="12" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6890-6909" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6890-6909" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6890-6909" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6890-6909" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="13" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="6405-6427" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6405-6427" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="6405-6427" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6405-6427" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="14" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="15" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="6527-6546" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6527-6546" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="6527-6546" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6527-6546" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="3:31:32">2026.1.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  f       §        §XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒßa�4½KðQ³à9ð¸€¾ÚkZŠ=j {ç×ÙwªuJt•�ÔY¶ë^‰ÓÀÔaf¹º¾ÑÙïdf–fº"kŠvÖÕJ¡HiR›Ø5h fRugæ˜  QQøÙ�P!´¦P:ƒEº�:ªºã•m8�Š«j�V Ó­Ô5.µ;b;µ¯?ÿùržó YCÂ#*.f)ª÷Ù£Ëªb?~âë¡ÄÚù º¿îÏT5Š,…4¯¸ë¡GW\¸“ê44Áýø1Ð�¸ÃÕOr&Q�øÕ7_ô�rˆJJ TKVïµ2k]@8PcôPŸö‰€n€>Â7c©ØŠ’Ä)U\¹ÿÜÈ*¾¡UÜeŸ`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%L<Ç¹ÇãCcS­	<9r—_	û‘y““c5jTÎ±•eQªjK½P XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'ò&õ•þèÒ�¼z »8¯ .û„†‡ŸËL º¶]*èR�]®$ÅšR[áíi=â¨[ÝžõžøVJÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@       ¦   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  7„<?xml version="1.0"?><Features recycledIDs="17" nextValidID="32"><Feature recentID="31" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6148-6252" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6078" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5287-6078" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6079-6147" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4174-4180" color="#ffffff" type="standard"/><Segment range="4181-4198" color="#ffffff" type="standard"/><Segment name="-35" range="4199-4204" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4219-4240" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4150-4166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4528-5116" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2593-2595" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2596-2598" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2599-3312" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="3312" maxFusedRunOn="3312" detectionMode="exactProteinMatch"><Segment range="2593-2595" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2596-2598" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2599-3309" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3474-3988" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2007-2009" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="18" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2007-2009" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="16" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1451" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="15" name="mut" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1992-1992" color="#a6acb3" type="standard"/></Feature><Feature recentID="14" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1452-2006" color="#ffffff" type="standard"/><Q name="chromosome"><V text="7"/></Q><Q name="db_xref"><V text="9606" predef="taxon"/></Q><Q name="map"><V text="7q36.1"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Homo sapiens"/></Q></Feature><Feature recentID="13" name="RHEB" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1452-2006" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog, mTORC1 binding"/></Q></Feature><Feature recentID="12" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1452-1503" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="11" name="RHEB" directionality="1" translationMW="20481.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1452-2006" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="3.6.5.2"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS5927.1" predef="CCDS"/><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog enriched in brain 2"/></Q><Q name="product"><V text="GTP-binding protein Rheb"/></Q><Q name="protein_id"><V text="NP_005605.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1554-1580" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q15382.1);&#10;Region: Effector region. /evidence=ECO:0000305"/></Q></Feature><Feature recentID="9" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1554-1556" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Important for autoinhibition of GTPase activity. /evidence=ECO:0000269|PubMed:22819219; propagated from UniProtKB/Swiss-Prot (Q15382.1); other site"/></Q></Feature><Feature recentID="8" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1839-1841" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Phosphoserine, by MAPKAPK5. /evidence=ECO:0000250|UniProtKB:Q921J2; propagated from UniProtKB/Swiss-Prot (Q15382.1); phosphorylation site"/></Q></Feature><Feature recentID="7" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1993-1994" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Cysteine methyl ester. /evidence=ECO:0000269|PubMed:12906785, ECO:0000269|PubMed:15308774, ECO:0000269|PubMed:22002721, ECO:0007744|PDB:3T5G; propagated from UniProtKB/Swiss-Prot (Q15382.1); methylation site"/></Q></Feature><Feature recentID="6" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1504-1575" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="5" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1576-1643" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="4" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1644-1726" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="3" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1727-1783" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1784-1831" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1832-1913" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="0" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1914-2006" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature></Features>
  #<?xml version="1.0"?><Primers nextValidID="16"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="R" sequence="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-09-25T02:36:27Z"><BindingSite location="1968-2016" boundStrand="1" annealedBases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" meltingTemperature="73"><Component hybridizedRange="1968-2016" bases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg"/></BindingSite><BindingSite simplified="1" location="1968-2016" boundStrand="1" annealedBases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" meltingTemperature="73"><Component hybridizedRange="1968-2016" bases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg"/></BindingSite></Primer><Primer recentID="1" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5909-5928" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5909-5928" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5909-5928" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5909-5928" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="2" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="3245-3266" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3245-3266" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="3245-3266" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3245-3266" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="3" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2637-2658" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2637-2658" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2637-2658" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2637-2658" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="4" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2898-2917" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2898-2917" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2898-2917" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2898-2917" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="5" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="2184-2201" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2184-2201" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="2184-2201" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2184-2201" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="6" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="2411-2430" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2411-2430" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="2411-2430" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2411-2430" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="7" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="4356-4373" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4356-4373" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="4356-4373" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4356-4373" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="8" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6710-6732" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6710-6732" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6710-6732" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6710-6732" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="9" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="10" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4607-4626" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4607-4626" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4607-4626" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4607-4626" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="11" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="6319-6337" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6319-6337" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="6319-6337" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6319-6337" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="12" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6860-6879" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6860-6879" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6860-6879" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6860-6879" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="13" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="6375-6397" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6375-6397" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="6375-6397" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6375-6397" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="14" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="15" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="6497-6516" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6497-6516" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="6497-6516" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6497-6516" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="3:30:39">2026.1.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  h     
  ¿        ¿XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`yÔq‡Q�4½KðQ³à9ð¸€¾ÚkZŠ=j {ç×ÙwªuJt•�ÔY¶ë^‰ÓÀÔaf¹º¾ÑÙïdf–fº"kŠvÖÕJ¡HiR›Ø5h fRugæ˜  QQøÙ�P!´¦P:ƒEº�:ªºã•m8�Š«j�V Ó­Ô5.µ;b;µ¯?ÿùržó YCÂ#*.f)ª÷Ù£Ëªb?~âë¡ÄÚù º¿îÏT5Š,…4¯¸ë¡GW\¸“ê44Áýø1Ð�¸ÃÕOr&Q�øÕ7_ô�rˆJJ TKVïµ2k]@8PcôPŸö‰€n€>Â7c©ØŠ’Ä)U\¹ÿÜÈ*¾¡UÜeŸ`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%L<Ç¹ÇãCcS­	<9r—_	û‘y““c5jTÎ±•eQªjK½P XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'ò&õ•þèÒ�¼z »8¯ .û„†‡ŸËL º¶]*èR�]®$ÅšR[áíi=â¨[ÝžõžøVJÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@       ¾   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  9ƒ<?xml version="1.0"?><Features nextValidID="32"><Feature recentID="31" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6172-6276" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6102" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5311-6102" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6103-6171" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4198-4204" color="#ffffff" type="standard"/><Segment range="4205-4222" color="#ffffff" type="standard"/><Segment name="-35" range="4223-4228" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="28" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4243-4264" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4174-4190" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4552-5140" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2617-2619" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2620-2622" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2623-3336" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="3336" maxFusedRunOn="3336" detectionMode="exactProteinMatch"><Segment range="2617-2619" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2620-2622" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2623-3333" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="23" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3498-4012" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2031-2033" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="18" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2031-2033" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="17" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2030" maxFusedRunOn="2030" detectionMode="exactProteinMatch"><Segment range="1452-1475" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="16" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1452" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="15" name="mut" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2016-2016" color="#a6acb3" type="standard"/></Feature><Feature recentID="14" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1476-2030" color="#ffffff" type="standard"/><Q name="chromosome"><V text="7"/></Q><Q name="db_xref"><V text="9606" predef="taxon"/></Q><Q name="map"><V text="7q36.1"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Homo sapiens"/></Q></Feature><Feature recentID="13" name="RHEB" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1476-2030" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog, mTORC1 binding"/></Q></Feature><Feature recentID="12" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1476-1527" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="11" name="RHEB" directionality="1" translationMW="20481.46" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1476-2030" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="3.6.5.2"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS5927.1" predef="CCDS"/><V text="6009" predef="GeneID"/><V text="HGNC:10011" predef="HGNC"/><V text="601293" predef="MIM"/></Q><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Ras homolog enriched in brain 2"/></Q><Q name="product"><V text="GTP-binding protein Rheb"/></Q><Q name="protein_id"><V text="NP_005605.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1578-1604" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q15382.1);&#10;Region: Effector region. /evidence=ECO:0000305"/></Q></Feature><Feature recentID="9" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1578-1580" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Important for autoinhibition of GTPase activity. /evidence=ECO:0000269|PubMed:22819219; propagated from UniProtKB/Swiss-Prot (Q15382.1); other site"/></Q></Feature><Feature recentID="8" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1863-1865" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Phosphoserine, by MAPKAPK5. /evidence=ECO:0000250|UniProtKB:Q921J2; propagated from UniProtKB/Swiss-Prot (Q15382.1); phosphorylation site"/></Q></Feature><Feature recentID="7" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2017-2018" color="#a6acb3" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="note"><V text="Cysteine methyl ester. /evidence=ECO:0000269|PubMed:12906785, ECO:0000269|PubMed:15308774, ECO:0000269|PubMed:22002721, ECO:0007744|PDB:3T5G; propagated from UniProtKB/Swiss-Prot (Q15382.1); methylation site"/></Q></Feature><Feature recentID="6" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1528-1599" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="5" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1600-1667" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="4" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1668-1750" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="3" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1751-1807" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1808-1855" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1856-1937" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="0" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1938-2030" color="#708090" type="standard"/><Q name="gene"><V text="RHEB"/></Q><Q name="gene_synonym"><V text="RHEB2"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature></Features>
  #<?xml version="1.0"?><Primers nextValidID="16"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="R" sequence="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-09-25T02:36:27Z"><BindingSite location="1992-2040" boundStrand="1" annealedBases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" meltingTemperature="73"><Component hybridizedRange="1992-2040" bases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg"/></BindingSite><BindingSite simplified="1" location="1992-2040" boundStrand="1" annealedBases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg" meltingTemperature="73"><Component hybridizedRange="1992-2040" bases="cgctcgagctatcacatcaccgagcttgaagacttgccttgtgaagctg"/></BindingSite></Primer><Primer recentID="1" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5933-5952" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5933-5952" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5933-5952" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5933-5952" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="2" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="3269-3290" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3269-3290" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="3269-3290" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3269-3290" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="3" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2661-2682" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2661-2682" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2661-2682" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2661-2682" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="4" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2922-2941" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2922-2941" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2922-2941" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2922-2941" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="5" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="2208-2225" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2208-2225" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="2208-2225" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2208-2225" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="6" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="2435-2454" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2435-2454" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="2435-2454" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2435-2454" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="7" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="4380-4397" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4380-4397" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="4380-4397" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4380-4397" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="8" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6734-6756" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6734-6756" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6734-6756" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6734-6756" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="9" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="10" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4631-4650" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4631-4650" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4631-4650" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4631-4650" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="11" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="6343-6361" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6343-6361" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="6343-6361" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6343-6361" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="12" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6884-6903" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6884-6903" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6884-6903" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6884-6903" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="13" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="6399-6421" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6399-6421" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="6399-6421" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6399-6421" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="14" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="15" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="6521-6540" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6521-6540" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="6521-6540" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6521-6540" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="3:30:35">2026.1.26</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  Lw       ”        ”XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`yÔq‡RNÄÀmkÏÿþ\§ƒÅ<èPðˆÊ‹™Šj½öhòêƒX„Á_ƒøºèq6¾@.¯û³Õ�Bâ…‹!AM+î:èQÕ×.$Çú�0_ÿ~t#î0õSÜ‰”g~5C×ý#Ü¢�’ˆÕƒ»íLš×Pý'ý¢`†à°�Øêv"¤±
UW.CÿÅ÷ r
¯¨UwgØ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IS1îD±øÐØÔëBO\¥×Â~ä^däØÍZ•3†leYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾’¶˜3EDàZ!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇÔØÔƒ?]/ü=À>=ösÅu3¶ñH1?¯öÂ°Æd3Ó_7âð†<=Æ2ðÉÆ’õ¨¥GA›H/C¹*‡µÖmôáO™q=‘•eQªjK½P       “   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !,<?xml version="1.0"?><Features recycledIDs="0,1,2,3" nextValidID="20"><Feature recentID="19" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1452" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="18" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1478" maxFusedRunOn="1478" detectionMode="exactProteinMatch"><Segment range="1452-1475" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1476-1478" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1476-1478" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="15" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2943-3457" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="2781" maxFusedRunOn="2781" detectionMode="exactProteinMatch"><Segment range="2062-2064" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2065-2067" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2068-2778" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2062-2064" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2065-2067" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2068-2781" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3997-4585" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3619-3635" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3688-3709" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3643-3649" color="#ffffff" type="standard"/><Segment range="3650-3667" color="#ffffff" type="standard"/><Segment name="-35" range="3668-3673" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5547" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4756-5547" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5548-5616" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5617-5721" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   N<?xml version="1.0"?><Primers recycledIDs="1,0" nextValidID="17"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5378-5397" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5378-5397" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5378-5397" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5378-5397" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="3" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="2714-2735" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="2714-2735" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="2714-2735" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="2714-2735" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="4" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2106-2127" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2106-2127" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2106-2127" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2106-2127" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="5" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2367-2386" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2367-2386" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2367-2386" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2367-2386" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="6" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="1653-1670" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="1653-1670" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="1653-1670" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="1653-1670" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="7" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="1880-1899" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="1880-1899" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="1880-1899" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="1880-1899" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="8" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="3825-3842" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3825-3842" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="3825-3842" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3825-3842" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="9" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6179-6201" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6179-6201" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6179-6201" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6179-6201" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="10" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="11" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4076-4095" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4076-4095" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4076-4095" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4076-4095" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="12" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="5788-5806" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="5788-5806" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="5788-5806" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="5788-5806" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="13" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6329-6348" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6329-6348" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6329-6348" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6329-6348" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="14" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="5844-5866" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="5844-5866" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="5844-5866" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="5844-5866" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="15" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="16" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="5966-5985" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="5966-5985" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="5966-5985" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="5966-5985" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="2:32:54">2025.9.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" journal="Nat Protoc. 2006;1(1):406-17." title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263"/>
</References>
</Notes>
  XU     	  ¼        ¼XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`yÔq‡Q�4½KðQ³à9ð¸€¾ÚkZŠ=j {ç×ÙwªuJt•�ÔY¶ë^‰ÓÀäaf¹º¾ÑÙïdf–fº"kŠvÖÕJ¡HiR›Ø5h fRugæ˜  QQøÙ�P!´¦P:ƒEº�:ªºã•m8�Š«j�V Ó­Ô5.¶;bNÄÀmkÏÿþ\§ƒÅ<èPðˆÊ‹™Šj½öhòêƒX„Á_ƒøºèq6¾@.¯û³Õ�Bâ…‹!AM+î:èQÕ×.$Çú�0_ÿ~t#î0õSÜ‰”g~5C×ý#Ü¢�’ˆÕƒ»íLš×Pý'ý¢`†à°�Øêv"¤±
UW.CÿÅ÷ r
¯¨UwgØ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IS1îD±øÐØÔëBO\¥×Â~ä^däØÍZ•3†leYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾’¶˜3EDàZ!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇÔØÔƒ?]/ü=À>=ösÅu3¶ñH1?¯öÂ°Æd3Ó_7âð†<=Æ2ðÉÆ’õ¨¥GA›H/C¹*‡µÖmôáO™q=‘•eQªjK½P       »   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &�<?xml version="1.0"?><Features nextValidID="20"><Feature recentID="3" name="RHEB-Q64L" directionality="1" translationMW="20482.56" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2030" maxFusedRunOn="2030"><Segment range="1476-2027" color="#ffff99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="19" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1452" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="18" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2030" maxFusedRunOn="2030" detectionMode="exactProteinMatch"><Segment range="1452-1475" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="17" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2028-2030" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="16" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2028-2030" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="15" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3495-4009" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="3333" maxFusedRunOn="3333" detectionMode="exactProteinMatch"><Segment range="2614-2616" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2617-2619" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2620-3330" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2614-2616" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2617-2619" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2620-3333" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4549-5137" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4171-4187" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4240-4261" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4195-4201" color="#ffffff" type="standard"/><Segment range="4202-4219" color="#ffffff" type="standard"/><Segment name="-35" range="4220-4225" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6099" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5308-6099" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6100-6168" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="4" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6169-6273" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="mutant" directionality="1" translationMW="174.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2030" maxFusedRunOn="2030"><Segment range="1956-1958" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="1" name="mutant" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2030" maxFusedRunOn="2030"><Segment range="1665-1667" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="mutant" directionality="1" translationMW="149.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2030" maxFusedRunOn="2030"><Segment range="1983-1985" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
  &?<?xml version="1.0"?><Primers nextValidID="17"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="F" sequence="gcggatcccaattggccaccatggactacaaggatgacgatgacaagatgccgcagtccaagtcccggaag" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-09-24T03:02:14Z"><BindingSite location="1428-1498" boundStrand="0" annealedBases="gcggatcccaattggccaccatggactacaaggatgacgatgacaagatgccgcagtccaagtcccggaag" meltingTemperature="78"><Component hybridizedRange="1428-1498" bases="gcggatcccaattggccaccatggactacaaggatgacgatgacaagatgccgcagtccaagtcccggaag"/></BindingSite><BindingSite simplified="1" location="1428-1498" boundStrand="0" annealedBases="gcggatcccaattggccaccatggactacaaggatgacgatgacaagatgccgcagtccaagtcccggaag" meltingTemperature="78"><Component hybridizedRange="1428-1498" bases="gcggatcccaattggccaccatggactacaaggatgacgatgacaagatgccgcagtccaagtcccggaag"/></BindingSite></Primer><Primer recentID="1" name="R" sequence="ctcgagctacatcaccgagcatgaagacttgccttgtgaagctgcccc" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-09-24T02:54:58Z"><BindingSite location="1988-2035" boundStrand="1" annealedBases="ctcgagctacatcaccgagcatgaagacttgccttgtgaagctgcccc" meltingTemperature="74"><Component hybridizedRange="1988-2035" bases="ctcgagctacatcaccgagcatgaagacttgccttgtgaagctgcccc"/></BindingSite><BindingSite simplified="1" location="1988-2035" boundStrand="1" annealedBases="ctcgagctacatcaccgagcatgaagacttgccttgtgaagctgcccc" meltingTemperature="74"><Component hybridizedRange="1988-2035" bases="ctcgagctacatcaccgagcatgaagacttgccttgtgaagctgcccc"/></BindingSite></Primer><Primer recentID="2" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5930-5949" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5930-5949" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5930-5949" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5930-5949" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="3" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="3266-3287" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3266-3287" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="3266-3287" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3266-3287" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="4" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2658-2679" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2658-2679" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2658-2679" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2658-2679" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="5" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2919-2938" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2919-2938" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2919-2938" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2919-2938" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="6" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="2205-2222" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2205-2222" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="2205-2222" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2205-2222" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="7" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="2432-2451" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2432-2451" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="2432-2451" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2432-2451" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="8" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="4377-4394" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4377-4394" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="4377-4394" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4377-4394" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="9" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6731-6753" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6731-6753" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6731-6753" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6731-6753" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="10" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="11" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4628-4647" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4628-4647" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4628-4647" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4628-4647" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="12" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="6340-6358" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6340-6358" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="6340-6358" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6340-6358" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="13" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6881-6900" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6881-6900" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6881-6900" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6881-6900" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="14" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="6396-6418" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6396-6418" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="6396-6418" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6396-6418" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="15" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="16" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="6518-6537" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6518-6537" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="6518-6537" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6518-6537" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="2:32:48">2025.9.25</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" journal="Nat Protoc. 2006;1(1):406-17." title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263"/>
</References>
</Notes>
  WÝ     
  ½        ½XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`yÔq‡Q�4½KðQ³à9ð¸€¾ÚkZŠ=j {ç×ÙwªuJt•�ÔY¶ë^‰ÓÀäaf¹º¾ÑÙïdf–fº"kŠvÖÕJ¡HiR›Ø5h fRugæ˜  QQøÙ�P!´¦P:ƒEº�:ªºã•m8�Š«j�V Ó­Ô5.¶;c“±0Zóÿÿ—)àñO:”<"2¢æbš¯}š<º Ö!ðWàþ.ºM¯�«þìõAcP¸¡bÈPSJû�NºuuË‰1þ£CLÿßƒûŒ=T÷"eß�PÃuÿH÷(„ d¢Dµ`îûS&µÔ…?E	ÿh˜á¸ì#v:�ˆ©,B•UË�ÿñ}À‚«ê]ÆYöM@ÄŠß ‚?o‚ÄàpÇ%p÷R´È¼11�~pÔáþ­¸ß|58ü�àýþÈ}þœ24Ž´óŸñ÷aM7ºÔ¼ö?9ËÐLßnºÔp×�Gó<B¬@Ç‚Í±8P6Ç­Avøu8uç]=×.fØ<u4QpÛP…ëQ¼÷]±24ìñ÷ŸMöÃ?Èã�Ç_yøMý/?„ÕõÄS1·`¾8²<ð{°ØN'RTÃÌ{‘,~465:Ð“Ã—)uðŸ¹™963V¥Lá›VU¦¤»ÑU …Gýø�¨59:R\ö¨Ô6YÙxE‘­P¤DGM`=]n ”Ò¾?Ã´Qt`¿ÑŒ/Ïí4«‘ÛF*ôÿP~‡ø�¦¡y}m+:ìëŠÌÎ¸ïñ;YTOÝíì3=/¼dxË?"oY_î�(ÛÇ¢³Šúï¸Hhyü´À«`%Ò®…(uÚáA,Y¥%¾Ö“Þ*…½ÙïYï…d­¦LÑQ8ˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“*ª*54Ñ§3EUA»QFú†ê¹À.í \UíÊP ¶¦ÕPn·äoªV•XR©[[•&fW ¸t§Ö:[HC~nÓ‹š²¶öJ>ÿ,‰yÆp:}¸?Ò8ÖóýÎßôjm5•ôôðP<LÑH/�z›Ï½½.ih£ÀS‘%$¬ô¥’£5Ê(öŽt6È"Üì² ˜:ÚÓ¼+õÆÔ1)ØoýŠ#US
NúÂûÆÊ-” óH¦ÞØ)ƒMãe®éâØöòQŽ«ˆx!'µõ-®d‰Œ1ñ(î�Ã-g–|ÏvM©TŽÛhrºÀ1W»PnŸ8¡Ò±‰{#^­6êž­ôÊ¸MUÐPÕcÍU@Y@ÇpV(Yížëê­fšÚ›@¦‹¶vj&¤Ueud¼ÍÚ¿ÅR=øråöšm‡i~eVC&ÜCú²ìÌ«aÂ~áÝ�;ðGtè¸”Á�4u?-2Ó(0"À8:^c´M'„Hßf0ˆV|ÝÉAgÍ´3ÚÏkCŒ×Š@ÆÂ0>ë9§=81PˆãPÆ” %Ï@ªý-Ç(•qÃ5‚65`Ï×Kÿp�}ŸÜñ]Lí„üROÆëý°†,1™ô×Íøƒ<!�qŒ¼2D1¤½j)QÐfÒÐîJ¡íu›}8SæDœOdeYTjš’ïET        ¼   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ,<?xml version="1.0"?><Features recycledIDs="3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,22" nextValidID="41"><Feature recentID="40" name="mutant" directionality="1" translationMW="149.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2063" maxFusedRunOn="2063"><Segment range="1983-1985" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="mutant" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2063" maxFusedRunOn="2063"><Segment range="1665-1667" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="38" name="mutant" directionality="1" translationMW="174.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2063" maxFusedRunOn="2063"><Segment range="1956-1958" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="RHEB-Q64L" directionality="1" translationMW="20482.56" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2063" maxFusedRunOn="2063"><Segment range="1476-2027" color="#ffff99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="Fntb" directionality="1" translationMW="-1.00" type="CDS" allowSegmentOverlaps="0" readingFrame="2" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="438" hitsStopCodon="1"><Segment range="2028-2031" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="2.5.1.58"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS25998.1" predef="CCDS"/><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="isoform 1 is encoded by transcript variant 1;&#10;protein farnesyltransferase subunit beta; FTase-beta; CAAX farnesyltransferase subunit beta; ras proteins prenyltransferase subunit beta"/></Q><Q name="product"><V text="protein farnesyltransferase subunit beta isoform&#10;1"/></Q><Q name="protein_id"><V text="NP_666039.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="36" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6170-6274" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6100" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5309-6100" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6101-6169" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="34" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4196-4202" color="#ffffff" type="standard"/><Segment range="4203-4220" color="#ffffff" type="standard"/><Segment name="-35" range="4221-4226" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4241-4262" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4172-4188" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4550-5138" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2615-2617" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2618-2620" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2621-3334" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="3334" maxFusedRunOn="3334" detectionMode="exactProteinMatch"><Segment range="2615-2617" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2618-2620" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2621-3331" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3496-4010" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2028-2031" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="23" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2028-2031" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2031" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2063" maxFusedRunOn="2063" detectionMode="exactProteinMatch"><Segment range="1452-1475" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1452" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   N<?xml version="1.0"?><Primers recycledIDs="1,0" nextValidID="17"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="2" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5931-5950" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5931-5950" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5931-5950" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5931-5950" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="3" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="3267-3288" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3267-3288" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="3267-3288" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3267-3288" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="4" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2659-2680" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2659-2680" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2659-2680" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2659-2680" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="5" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2920-2939" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2920-2939" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2920-2939" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2920-2939" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="6" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="2206-2223" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2206-2223" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="2206-2223" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2206-2223" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="7" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="2433-2452" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2433-2452" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="2433-2452" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="2433-2452" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="8" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="4378-4395" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4378-4395" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="4378-4395" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="4378-4395" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="9" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6732-6754" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6732-6754" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6732-6754" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6732-6754" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="10" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="11" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4629-4648" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4629-4648" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4629-4648" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4629-4648" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="12" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="6341-6359" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6341-6359" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="6341-6359" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="6341-6359" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="13" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6882-6901" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6882-6901" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6882-6901" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="6882-6901" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="14" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="6397-6419" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6397-6419" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="6397-6419" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="6397-6419" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="15" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="16" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="6519-6538" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6519-6538" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="6519-6538" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="6519-6538" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="8:53:46">2025.9.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" pubMedID="17406263" title="Generation of T-cell receptor retrogenic mice." journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  y[     Q   Û         ÛXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`yÔq‡Q�4½KðQ³à9ð¸€¾ÚkZŠ=j {ç×ÙwªuJt•�ÔY¶ë^‰ÓÀäaf¹º¾ÑÙïdf–fº"kŠvÖÕJ¡HiR›Ø5h fRugæ˜  QQøÙ�P!´¦P:ƒEº�:ªºã•m8�Š«j�V Ó­Ô5.¶;aƒ®±+ë~i¢ûÛ¯¿â ´_8™.øMÈÀL(Ôqí WKvöEÐõIEÐBëJéÔ©}ú'Ô;j�MëÚnD>±×‡F>a"àcÓÏø;Žxãtø¾‡öÿÑ’tr"Ò¾â-ôpƒ¨ü-'÷~Íýé�<µŒâ6ÛÑ>uËitW¾´š‰ïåM;ªí�Ë!”6xŽ>ÂO»·×m·½º…ÃÅ�“Ð�Eà0Ú=�OÜ ç~¸ƒ ã8)¾ÔP\®¡~NŽu€‡SÃF0¨PC«@ó�u8 pãžúàÐÐ;ãþ8ßûÜíBãûM„ö�ŠöÓC8ÐIÛáˆãÒ/O ¸OƒRÜ‡®mžŽxøL>ïhý6¬Á=Š7‚Ø�Å\®4ýÝþ"uÚá!½}ÝêãE=/ÐêX…B´o�|y;µ¯?ÿùržó YCÂ#*.f)ª÷Ù£Ëªb?~âë¡ÄÚù º¿îÏT5Š,…4¯¸ë¡GW\¸“ê44Áýø1Ð�¸ÃÕOr&Q�øÕ7_ô�rˆJJ TKVïµ2k]@8PcôPŸö‰€n€>Â7c©ØŠ’Ä)U\¹ÿÜÈ*¾¡UÜeŸ`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%L<Ç¹ÇãCcS­	<9r—_	û‘y““c5jTÎ±•eQªjK½P XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'ò&õ•þèÒ�¼z »8¯ .û„†‡ŸËL º¶]*èR�]®$ÅšR[áíi=â¨[ÝžõžøVJÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@        Ú   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  F <?xml version="1.0"?><Features nextValidID="41"><Feature recentID="40" name="mutant" directionality="1" translationMW="149.21" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3341" maxFusedRunOn="3341"><Segment range="1983-1985" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="39" name="mutant" directionality="1" translationMW="131.17" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3341" maxFusedRunOn="3341"><Segment range="1665-1667" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="38" name="mutant" directionality="1" translationMW="174.20" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3341" maxFusedRunOn="3341"><Segment range="1956-1958" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="37" name="RHEB-Q64L" directionality="1" translationMW="20482.56" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3341" maxFusedRunOn="3341"><Segment range="1476-2027" color="#ffff99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="21" name="Fntb" directionality="1" translationMW="48819.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2028-3341" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="2.5.1.58"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS25998.1" predef="CCDS"/><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="isoform 1 is encoded by transcript variant 1;&#10;protein farnesyltransferase subunit beta; FTase-beta; CAAX farnesyltransferase subunit beta; ras proteins prenyltransferase subunit beta"/></Q><Q name="product"><V text="protein farnesyltransferase subunit beta isoform&#10;1"/></Q><Q name="protein_id"><V text="NP_666039.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="36" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7480-7584" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="7410" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6619-7410" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="7411-7479" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="34" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5506-5512" color="#ffffff" type="standard"/><Segment range="5513-5530" color="#ffffff" type="standard"/><Segment name="-35" range="5531-5536" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5551-5572" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5482-5498" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5860-6448" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3925-3927" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3928-3930" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3931-4644" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4644" maxFusedRunOn="4644" detectionMode="exactProteinMatch"><Segment range="3925-3927" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="3928-3930" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3931-4641" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4806-5320" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2028-3341" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="23" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="2028-3341" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="22" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2028-2171" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="20" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2331-2333" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Important for selectivity against geranylgeranyl diphosphate. /evidence=ECO:0000250|UniProtKB:P49356; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); other site"/></Q></Feature><Feature recentID="19" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2394-2519" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 1"/></Q></Feature><Feature recentID="18" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2547-2672" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 2"/></Q></Feature><Feature recentID="17" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2691-2816" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 3"/></Q></Feature><Feature recentID="16" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2835-2963" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 4"/></Q></Feature><Feature recentID="15" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3021-3149" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 5"/></Q></Feature><Feature recentID="14" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3321-3323" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0007744|PubMed:21183079; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); phosphorylation site"/></Q></Feature><Feature recentID="13" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3333-3335" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Phosphothreonine.&#10;/evidence=ECO:0007744|PubMed:21183079; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); phosphorylation site"/></Q></Feature><Feature recentID="12" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2172-2236" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="11" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2237-2309" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="10" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2310-2401" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="9" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2402-2548" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="8" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2549-2632" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="7" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2633-2719" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="6" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2720-2849" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="5" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2850-2982" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="4" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2983-3094" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="3" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3095-3209" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3210-3341" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="3341" maxFusedRunOn="3341" detectionMode="exactProteinMatch"><Segment range="1452-1475" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1452" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
  %ì<?xml version="1.0"?><Primers nextValidID="17"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="NEW Fntb R1" sequence="CCGctcgagctagtcggtggcaggatctgaggtcac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-03-31T10:30:13Z"><BindingSite location="3314-3349" boundStrand="1" annealedBases="CCGctcgagctagtcggtggcaggatctgaggtcac" meltingTemperature="72"><Component hybridizedRange="3314-3349" bases="CCGctcgagctagtcggtggcaggatctgaggtcac"/></BindingSite><BindingSite simplified="1" location="3314-3349" boundStrand="1" annealedBases="CCGctcgagctagtcggtggcaggatctgaggtcac" meltingTemperature="72"><Component hybridizedRange="3314-3349" bases="CCGctcgagctagtcggtggcaggatctgaggtcac"/></BindingSite></Primer><Primer recentID="1" name="new Fntb F1" sequence="cgcaattggccaccatggactacaaggatgacgatgacaagatggcttcttcgagttccttcacct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-03-31T10:29:12Z"><BindingSite location="2026-2051" boundStrand="0" annealedBases="gatggcttcttcgagttccttcacct" meltingTemperature="63"><Component bases="cgcaattggccaccatggactacaaggatgacgatgacaa"/><Component hybridizedRange="2026-2051" bases="gatggcttcttcgagttccttcacct"/></BindingSite><BindingSite simplified="1" location="2026-2051" boundStrand="0" annealedBases="gatggcttcttcgagttccttcacct" meltingTemperature="63"><Component bases="cgcaattggccaccatggactacaaggatgacgatgacaa"/><Component hybridizedRange="2026-2051" bases="gatggcttcttcgagttccttcacct"/></BindingSite></Primer><Primer recentID="2" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="7241-7260" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="7241-7260" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="7241-7260" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="7241-7260" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="3" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="4577-4598" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="4577-4598" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="4577-4598" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="4577-4598" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="4" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="3969-3990" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="3969-3990" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="3969-3990" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="3969-3990" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="5" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="4230-4249" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="4230-4249" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="4230-4249" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="4230-4249" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="6" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="3516-3533" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="3516-3533" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="3516-3533" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="3516-3533" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="7" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="3743-3762" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="3743-3762" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="3743-3762" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="3743-3762" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="8" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="5688-5705" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="5688-5705" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="5688-5705" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="5688-5705" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="9" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="8042-8064" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="8042-8064" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="8042-8064" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="8042-8064" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="10" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="11" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="5939-5958" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5939-5958" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="5939-5958" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5939-5958" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="12" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="7651-7669" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="7651-7669" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="7651-7669" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="7651-7669" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="13" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="8192-8211" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="8192-8211" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="8192-8211" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="8192-8211" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="14" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="7707-7729" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="7707-7729" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="7707-7729" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="7707-7729" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="15" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="16" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="7829-7848" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="7829-7848" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="7829-7848" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="7829-7848" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="8:53:28">2025.9.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" pubMedID="17406263" title="Generation of T-cell receptor retrogenic mice." journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  sŸ     Ç  ³        ³XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖƒß`yÔq‡Qƒ®±+ë~i¢ûÛ¯¿â ´_8™.øMÈÀL(Ôqí WKvöEÐõIEÐBëJéÔ©}ú'Ô;j�MëÚnD>±×‡F>a"àcÓÏø;Žxãtø¾‡öÿÑ’tr"Ò¾â-ôpƒ¨ü-'÷~Íýé�<µŒâ6ÛÑ>uËitW¾´š‰ïåM;ªí�Ë!”6xŽ>ÂO»·×m·½º…ÃÅ�“Ð�Eà0Ú=�OÜ ç~¸ƒ ã8)¾ÔP\®¡~NŽu€‡SÃF0¨PC«@ó�u8 pãžúàÐÐ;ãþ8ßûÜíBãûM„ö�ŠöÓC8ÐIÛáˆãÒ/O ¸OƒRÜ‡®mžŽxøL>ïhý6¬Á=Š7‚Ø�Å\®4ýÝþ"uÚá!½}ÝêãE=/ÐêX…B´o�|y;µ¯?ÿùržó YCÂ#*.f)ª÷Ù£Ëªb?~âë¡ÄÚù º¿îÏT5Š,…4¯¸ë¡GW\¸“ê44Áýø1Ð�¸ÃÕOr&Q�øÕ7_ô�rˆJJ TKVïµ2k]@8PcôPŸö‰€n€>Â7c©ØŠ’Ä)U\¹ÿÜÈ*¾¡UÜeŸ`„ÔA8­ð#öø,NrWu+L‹Ã1÷çNáJÛ�÷ÃS�Èþßì‡ßáùÃ#HëO9ÿvÓqë­KÏcñCœ½Íöë­AÇyÔ3Ä!JÄwø!|Û…lzÔp×o€7S�'^uÓÝræmƒÇSEµ^µÏuÛpÓ#NÏyôÑwÿl1ÃüŽ8üu÷Ÿ„ßÒóøMQÿ\E3vã�+!óÏ·»�Äâu%L<Ç¹ÇãCcS­	<9r—_	û‘y““c5jTÎ±•eQªjK½P XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'ò&õ•þèÒ�¼z »8¯ .û„†‡ŸËL º¶]*èR�]®$ÅšR[áíi=â¨[ÝžõžøVJÚ`tÍ€h…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0‚ª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡.Q_i¦Øv—æUd2mÄ?«.ÌÊ°†!WîØÓ¿p·N‹‰LðGSòÐ3-0"ƒ,ƒ¥æ0ÛDÑ¢xD�öc…gÍÑŒ”V|ÛC=¬ö´8Íx l#î³šsÓƒ Ž5iJ\ô
¯ÒÜr‰W3PX#cPFýt¿ð÷ ø÷ÙýÏÔÎØOÅ Äün¿ÛbÃ�ÏM|ßˆ3Âð÷ËÃ$CKÖ¢•m ½äª×Y·Ó…>dIÄöFU•F©©.ôU@       ²   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  A#<?xml version="1.0"?><Features nextValidID="37"><Feature recentID="21" name="Fntb" directionality="1" translationMW="48819.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1476-2789" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="2.5.1.58"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS25998.1" predef="CCDS"/><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="isoform 1 is encoded by transcript variant 1;&#10;protein farnesyltransferase subunit beta; FTase-beta; CAAX farnesyltransferase subunit beta; ras proteins prenyltransferase subunit beta"/></Q><Q name="product"><V text="protein farnesyltransferase subunit beta isoform&#10;1"/></Q><Q name="protein_id"><V text="NP_666039.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="36" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6928-7032" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="35" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6858" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6067-6858" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6859-6927" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="34" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4954-4960" color="#ffffff" type="standard"/><Segment range="4961-4978" color="#ffffff" type="standard"/><Segment name="-35" range="4979-4984" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="33" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4999-5020" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="32" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4930-4946" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="31" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5308-5896" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="30" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3373-3375" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3376-3378" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3379-4092" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="29" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4092" maxFusedRunOn="4092" detectionMode="exactProteinMatch"><Segment range="3373-3375" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="3376-3378" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3379-4089" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="28" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="27" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4254-4768" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="24" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1476-2789" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="23" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1476-2789" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="22" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1476-1619" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="20" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1779-1781" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Important for selectivity against geranylgeranyl diphosphate. /evidence=ECO:0000250|UniProtKB:P49356; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); other site"/></Q></Feature><Feature recentID="19" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1842-1967" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 1"/></Q></Feature><Feature recentID="18" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1995-2120" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 2"/></Q></Feature><Feature recentID="17" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2139-2264" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 3"/></Q></Feature><Feature recentID="16" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2283-2411" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 4"/></Q></Feature><Feature recentID="15" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2469-2597" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 5"/></Q></Feature><Feature recentID="14" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2769-2771" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0007744|PubMed:21183079; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); phosphorylation site"/></Q></Feature><Feature recentID="13" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2781-2783" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Phosphothreonine.&#10;/evidence=ECO:0007744|PubMed:21183079; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); phosphorylation site"/></Q></Feature><Feature recentID="12" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1620-1684" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="11" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1685-1757" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="10" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1758-1849" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="9" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1850-1996" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="8" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1997-2080" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="7" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2081-2167" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="6" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2168-2297" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="5" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2298-2430" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="4" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2431-2542" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="3" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2543-2657" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="2" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2658-2789" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="1" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2789" maxFusedRunOn="2789" detectionMode="exactProteinMatch"><Segment range="1452-1475" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAGÂ® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1452" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
  &8<?xml version="1.0"?><Primers nextValidID="17"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="NEW Fntb R1" sequence="CCGctcgagctagtcggtggcaggatctgaggtcac" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-03-31T10:30:13Z"><BindingSite location="2762-2797" boundStrand="1" annealedBases="CCGctcgagctagtcggtggcaggatctgaggtcac" meltingTemperature="72"><Component hybridizedRange="2762-2797" bases="CCGctcgagctagtcggtggcaggatctgaggtcac"/></BindingSite><BindingSite simplified="1" location="2762-2797" boundStrand="1" annealedBases="CCGctcgagctagtcggtggcaggatctgaggtcac" meltingTemperature="72"><Component hybridizedRange="2762-2797" bases="CCGctcgagctagtcggtggcaggatctgaggtcac"/></BindingSite></Primer><Primer recentID="1" name="new Fntb F1" sequence="cgcaattggccaccatggactacaaggatgacgatgacaagatggcttcttcgagttccttcacct" description="&lt;html&gt;&lt;body&gt;&#10;&#10;&lt;/body&gt;&lt;/html&gt;" dateAdded="2025-03-31T10:29:12Z"><BindingSite location="1436-1499" boundStrand="0" annealedBases="caattggccaccatggactacaaggatgacgatgacaagatggcttcttcgagttccttcacct" meltingTemperature="73"><Component bases="cg"/><Component hybridizedRange="1436-1499" bases="caattggccaccatggactacaaggatgacgatgacaagatggcttcttcgagttccttcacct"/></BindingSite><BindingSite simplified="1" location="1436-1499" boundStrand="0" annealedBases="caattggccaccatggactacaaggatgacgatgacaagatggcttcttcgagttccttcacct" meltingTemperature="73"><Component bases="cg"/><Component hybridizedRange="1436-1499" bases="caattggccaccatggactacaaggatgacgatgacaagatggcttcttcgagttccttcacct"/></BindingSite></Primer><Primer recentID="2" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="6689-6708" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="6689-6708" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="6689-6708" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="6689-6708" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="3" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="4025-4046" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="4025-4046" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="4025-4046" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="4025-4046" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="4" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="3417-3438" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="3417-3438" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="3417-3438" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="3417-3438" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="5" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="3678-3697" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="3678-3697" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="3678-3697" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="3678-3697" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="6" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="2964-2981" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2964-2981" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="2964-2981" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2964-2981" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="7" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="3191-3210" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="3191-3210" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="3191-3210" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="3191-3210" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="8" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="5136-5153" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="5136-5153" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="5136-5153" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="5136-5153" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="9" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="7490-7512" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="7490-7512" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="7490-7512" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="7490-7512" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="10" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="11" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="5387-5406" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5387-5406" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="5387-5406" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5387-5406" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="12" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="7099-7117" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="7099-7117" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="7099-7117" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="7099-7117" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="13" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="7640-7659" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="7640-7659" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="7640-7659" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="7640-7659" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="14" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="7155-7177" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="7155-7177" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="7155-7177" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="7155-7177" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="15" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="16" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="7277-7296" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="7277-7296" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="7277-7296" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="7277-7296" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="8:53:5">2025.9.23</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" pubMedID="17406263" title="Generation of T-cell receptor retrogenic mice." journal="Nat Protoc. 2006;1(1):406-17."/>
</References>
</Notes>
  ih     ¿  ’        ’XTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖ†ºÄ¯­ù¦‹ïn¾ÿŠ‚Ñ|âd¸Gá7#0£QÇ´�]-ÛÙCÕ$QoA­+§R¥÷èŸPíª4@E7¯i¸UúÇ^ù„‹��O?àî9ào�ÓàNú_ÛÿFH=ÑÈ‹Jøˆ´KÑÂ @ð´ŸÝû7÷¦4òÖ3ˆÛl7Dù×-¥Ð^úÒj'¿•5üpî«¶7,„ rPHÙâ8û	>îß]¶Þô~ê7OB=€PÃh ô?pƒ�úâƒŒà¦ûQArº…øQ:9ÖOÂ¡A­Î5Ôà�ÃŽ{ëƒC@ï�øã|Oïsµ�í6Ú+ÛMãA'o†#�H½< :àu>Kr¹¶z9ãá0û½£ôÚ°Sö(Üf`"?r¸Ó÷wø‰×h„P†ô=÷w«�ô¿C¨Aba~Ñ¾=ñäìLÖ¼ÿÿåÊx<SÎ�eŒ¨¹˜¦«ßf�.¨5ˆ@üø?‹®‡käêÿ»=PXÔ.(X²Ò¾àS®…]râL¨ÐÓÿ÷àÇB>ãU=È™FwãT0ÝÒ=Ê!((�Q-X;¾ÔÉ­u áA�ÑBÚ&¸n ûÝŽ§b*K¥Urä?ü_p ªú…Wq–}‚P1â·À �Ûà±81É\=Ô­2/@Äcßœ58…+n7ßN?#ø?²‡ç�#­<çü}ØSMÇ®µ/=�Årô7Û®µ5çQüÏ…+1ßà…ólN±ëPAÃ]¾ ÝN�y×OuË™¶M\6Ô!zÔo=×lAÃL�;<}çÓEßý°Çò8ãñ×Þ~KÏá5GýqÌmØ/Ž¬‡Ï<Þì6‰Ô•0óäK��N´$ðåÊ]|'îEæNMŒÕ©S8fÆU•F©©.ôU@aQÿ~$*NN”—=ª5�Vv^dE+FÔ)ÑÓE˜WA›ˆ%4¯�ðíÔ]/ôcóûM*äEöÑŠ½?Ô…aþ#é¨^_[JÎ»:â³3®;üNÖU÷_û{ÏKï2ÏÀŸÈ›ÖWû£J6ñè‚ìâ¾€@»î-0êØ	t«¡J]v¸@�KiIo‡µ¤÷Š¡ov{Ö{áY+i�Ó4TN E¢¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Â
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~¹E}¦›aÚ_™U�É·þ¬»3*Âp…_¸wcNüÂÝ:.%0cÀMOË@Ì´ÀŠ°—˜ÃmF‰á7ÙŒ"Ÿ7F2PEYómö³ÚÐã5â€P1°ŒºÎiÏNT b8Ô1¥(	sÐ*¿KqÊ%\pÍA`��A3õÒÿÃÜãßg÷<WS;a?ƒñºÿl!‹fC=5ó~ ÏcÃÜc/‘i/ZŠTt´‚ô;’¨{]fßNù‘'ÙVU¦¤»ÑU        ‘   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <ð<?xml version="1.0"?><Features nextValidID="35"><Feature recentID="34" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2625-2756" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="33" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2510-2624" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="32" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2398-2509" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="31" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2265-2397" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="30" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2135-2264" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="29" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2048-2134" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="28" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1964-2047" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="27" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1817-1963" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="26" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1725-1816" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="25" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1652-1724" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="24" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1587-1651" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="23" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2748-2750" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Phosphothreonine.&#10;/evidence=ECO:0007744|PubMed:21183079; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); phosphorylation site"/></Q></Feature><Feature recentID="22" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2736-2738" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Phosphoserine.&#10;/evidence=ECO:0007744|PubMed:21183079; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); phosphorylation site"/></Q></Feature><Feature recentID="21" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2436-2564" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 5"/></Q></Feature><Feature recentID="20" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2250-2378" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 4"/></Q></Feature><Feature recentID="19" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2106-2231" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 3"/></Q></Feature><Feature recentID="18" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1962-2087" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 2"/></Q></Feature><Feature recentID="17" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1809-1934" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="propagated from UniProtKB/Swiss-Prot (Q8K2I1.1);&#10;Region: PFTB 1"/></Q></Feature><Feature recentID="16" name="miscellaneous" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1746-1748" color="#a6acb3" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="Important for selectivity against geranylgeranyl diphosphate. /evidence=ECO:0000250|UniProtKB:P49356; propagated from UniProtKB/Swiss-Prot (Q8K2I1.1); other site"/></Q></Feature><Feature recentID="15" name="Fntb" directionality="1" translationMW="48819.85" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1443-2756" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="2.5.1.58"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="db_xref"><V text="CCDS25998.1" predef="CCDS"/><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="isoform 1 is encoded by transcript variant 1;&#10;protein farnesyltransferase subunit beta; FTase-beta; CAAX farnesyltransferase subunit beta; ras proteins prenyltransferase subunit beta"/></Q><Q name="product"><V text="protein farnesyltransferase subunit beta isoform&#10;1"/></Q><Q name="protein_id"><V text="NP_666039.1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="14" name="exon" type="exon" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1586" color="#708090" type="standard"/><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="inference"><V bool="1" text="2.1.0" predef="alignment"/></Q></Feature><Feature recentID="13" name="Fntb" directionality="1" type="gene" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1443-2756" color="#a6acb3" type="standard"/><Q name="db_xref"><V text="110606" predef="GeneID"/><V text="MGI:1861305" predef="MGI"/></Q><Q name="gene"><V text="Fntb"/></Q><Q name="gene_synonym"><V text="2010013E13Rik"/></Q><Q name="note"><V text="farnesyltransferase, CAAX box, beta"/></Q></Feature><Feature recentID="12" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1443-2756" color="#ffffff" type="standard"/><Q name="chromosome"><V text="12"/></Q><Q name="db_xref"><V text="10090" predef="taxon"/></Q><Q name="map"><V text="12 33.73 cM"/></Q><Q name="mol_type"><V predef="mRNA"/></Q><Q name="organism"><V text="Mus musculus"/></Q><Q name="strain"><V text="C57BL/6"/></Q></Feature><Feature recentID="11" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4221-4735" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="4059" maxFusedRunOn="4059" detectionMode="exactProteinMatch"><Segment range="3340-3342" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="3343-3345" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3346-4056" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="3340-3342" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="3343-3345" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="3346-4059" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5275-5863" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4897-4913" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4966-4987" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="4921-4927" color="#ffffff" type="standard"/><Segment range="4928-4945" color="#ffffff" type="standard"/><Segment name="-35" range="4946-4951" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6825" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6034-6825" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6826-6894" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6895-6999" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   :<?xml version="1.0"?><Primers nextValidID="15"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="6656-6675" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="6656-6675" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="6656-6675" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="6656-6675" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="3992-4013" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3992-4013" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="3992-4013" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="3992-4013" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="2" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="3384-3405" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="3384-3405" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="3384-3405" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="3384-3405" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="3" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="3645-3664" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="3645-3664" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="3645-3664" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="3645-3664" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="4" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="2931-2948" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2931-2948" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="2931-2948" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="57"><Component hybridizedRange="2931-2948" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="5" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="3158-3177" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="3158-3177" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="3158-3177" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="58"><Component hybridizedRange="3158-3177" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="6" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="5103-5120" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="5103-5120" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="5103-5120" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="5103-5120" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="7" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="7457-7479" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="7457-7479" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="7457-7479" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="7457-7479" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="8" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="9" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="5354-5373" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5354-5373" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="5354-5373" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5354-5373" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="10" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="7066-7084" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="7066-7084" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="7066-7084" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="7066-7084" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="11" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="7607-7626" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="7607-7626" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="7607-7626" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="57"><Component hybridizedRange="7607-7626" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="12" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="7122-7144" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="7122-7144" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="7122-7144" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="7122-7144" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="13" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="14" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="7244-7263" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="7244-7263" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="7244-7263" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="7244-7263" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="10:23:15">2025.3.31</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17." authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA"/>
</References>
</Notes>
  D€      v  p        pXTß‰
ƒS“¥%Ïj�C`U�—„YJÑµ
AtDtÑfÕÐfâ	M+ãü;@õFýÂüþÓJ¹}´b¯@�õáXˆúj—ÖÒ³®Î¸¬ÌëŽÿµ•Dý×þÞÃ3ÒûÆGŒ³ð'òkõ•>èâ£oÈÎè¾Ñ¡¡ã÷ì º¶ B½ñ�øý÷ÇÿóÀBSƒÓ*È¸»‹¨ŒŠˆðÛ–*3ã.'’“—“¸›ƒò‡¸]ü<×ørýê <ªˆ?„±ˆñÿ-b
à�G~UÒ¿>òáj£«
�|SÌË¢ã�3�6Êâ(»‹‡ˆ«‰¨¸U¤GŠ}ïéJ‡éx±0lí×ÒÂF-EÊŸ®;�_©rÁƒÌGú—Ä~ÛB”mBê·àüØýBÿ�²€Sè:¡ÿûøSú‰ßå>óïºûÛÏòîÿè_¾ÊÇüûïêoOíï®ä3ÁkFéÉ90oÖŽÄÀmkÏÿþ\§ƒÅ<èPðˆÊ‹™Šj½öhòêƒX„Á_ƒøºèq6¾@.¯û³Õ�Bâ…‹!AM+î:èQÕ×.$Çú�0_ÿ~t#î0õSÜ‰”g~5C×ý#Ü¢�’ˆÕƒ»íLš×Pý'ý¢`†à°�Øêv"¤±
UW.CÿÅ÷ r
¯¨UwgØ!5N+|ý¾�Ã•ÃÝJÓ"ðÄ@Æ=ùÃS‡øR¶ã}ðÔãò?ƒ÷û!÷ø~pÈÒ:ÓÎÇÝ…4ÜzëRóØüPç/A3}ºëPqÃ^uÌñR±þ_6Äá@Ûµ5ÛàÔàI×�t÷\¹›`ñÔÑEÃmB­FóÝvÄ4ÈÓ³ÇÞ}4]ÿÛpÿ#Ž?}çá7ô¼þT×LÆÝ‚øàJÈ|óÀmîÃ`q8�IS1îD±øÐØÔëBO\¥×Â~ä^däØÍZ•3†leYTjš’ïET ÷âB ÔäéIsÚ£PØgeáDR´mB�]4Y€õt¸‚SJøÿÐ=EÑ‚ÿF0¿?´Ò®D_m«Ð#ýAøVâ>š…åõ´¬ë³®+3:ã¿ÄíeQ?uÿ·°Ìô¾ñ‘ã,ü	ü‰½eº4£oˆ.Î+è¾á!¡çòÓ .­€—Jº E×k„	±f”–ø{ZOxª÷g½g¾’¶˜3EDàZ!Z)¼íÖ½Ývq<ÙR%O€#å„„åíÚZ2Œíãó«ÒÀWâÈôãial=s0C
Œš;¯:û;x{8ÎÂÊÃŒ10›NÞÕ%œ)½Ñm eÍEØ„ÕCÓUA|•Pó(àÊ«ÙóÿáÄÛuVÇ;RÑ1_ÇAæTgÐÊ¿øïìŒîø¯ãÎŸ~/>®þ°È3«¶NÜâBnÒ°‰+;Ôà8ˆÜ_ÿ+OÇÎ3é¼%æË¡/_ÂQÜzl÷ƒM=à—A¤Ñ1	‰#�º‚—œ9ÞEI¨&ã;Žô§ëTJ	;¡¼5u}ó‚L ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË”WÚi¶¥ù•Y›qêË³2¬!‡Uû‡v4ïÁ-Ó¢âS<ÑÔü´ËL À‹ àéyŒ6Ñ4hž#}˜Â!Yótc%UŸ6ÐÏk=­3^(Àû¬æœôàÅ@#�CR€—=«ô·¢UÇÔØÔƒ?]/ü=À>=ösÅu3¶ñH1?¯öÂ°Æd3Ó_7âð†<=Æ2ðÉÆ’õ¨¥GA›H/C¹*‡µÖmôáO™q=‘•eQªjK½P       o   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Q<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2907-3421" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="2745" maxFusedRunOn="2745" detectionMode="exactProteinMatch"><Segment range="2026-2028" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="2029-2031" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2032-2742" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="6" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="2026-2028" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="2029-2031" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="2032-2745" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="5" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3961-4549" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3583-3599" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3652-3673" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="3607-3613" color="#ffffff" type="standard"/><Segment range="3614-3631" color="#ffffff" type="standard"/><Segment name="-35" range="3632-3637" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5511" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4720-5511" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5512-5580" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5581-5685" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   :<?xml version="1.0"?><Primers nextValidID="15"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5342-5361" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5342-5361" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5342-5361" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5342-5361" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="2678-2699" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="2678-2699" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="2678-2699" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="2678-2699" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="2" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="2070-2091" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2070-2091" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="2070-2091" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="2070-2091" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="3" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="2331-2350" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2331-2350" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="2331-2350" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="2331-2350" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="4" name="IRES reverse" sequence="GCATTCCTTTGGCGAGAG" description="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev"><BindingSite location="1617-1634" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="56"><Component hybridizedRange="1617-1634" bases="GCATTCCTTTGGCGAGAG"/></BindingSite><BindingSite simplified="1" location="1617-1634" boundStrand="1" annealedBases="GCATTCCTTTGGCGAGAG" meltingTemperature="56"><Component hybridizedRange="1617-1634" bases="GCATTCCTTTGGCGAGAG"/></BindingSite></Primer><Primer recentID="5" name="IRES-F" sequence="TGGCTCTCCTCAAGCGTATT" description="IRES internal ribosome entry site, forward primer"><BindingSite location="1844-1863" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="57"><Component hybridizedRange="1844-1863" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite><BindingSite simplified="1" location="1844-1863" boundStrand="0" annealedBases="TGGCTCTCCTCAAGCGTATT" meltingTemperature="57"><Component hybridizedRange="1844-1863" bases="TGGCTCTCCTCAAGCGTATT"/></BindingSite></Primer><Primer recentID="6" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="3789-3806" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3789-3806" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="3789-3806" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="3789-3806" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="7" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6143-6165" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6143-6165" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6143-6165" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6143-6165" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="8" name="pBABE 5'" sequence="CTTTATCCAGCCCTCAC" description="Psi packaging signal, forward primer for pBABE vectors"><BindingSite location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite><BindingSite simplified="1" location="1372-1388" boundStrand="0" annealedBases="CTTTATCCAGCCCTCAC" meltingTemperature="51"><Component hybridizedRange="1372-1388" bases="CTTTATCCAGCCCTCAC"/></BindingSite></Primer><Primer recentID="9" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4040-4059" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4040-4059" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4040-4059" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4040-4059" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="10" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="5752-5770" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="5752-5770" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="5752-5770" boundStrand="0" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="5752-5770" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="11" name="pBRrevBam" sequence="GGTGATGTCGGCGATATAGG" description="pBR322 vectors, tet region, downstream of BamHI, reverse primer"><BindingSite location="6293-6312" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="56"><Component hybridizedRange="6293-6312" bases="GGTGATGTCGGCGATATAGG"/></BindingSite><BindingSite simplified="1" location="6293-6312" boundStrand="0" annealedBases="GGTGATGTCGGCGATATAGG" meltingTemperature="56"><Component hybridizedRange="6293-6312" bases="GGTGATGTCGGCGATATAGG"/></BindingSite></Primer><Primer recentID="12" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="5808-5830" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="5808-5830" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="5808-5830" boundStrand="1" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="5808-5830" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="13" name="pLXSN 5'" sequence="CCCTTGAACCTCCTCGTTCGACC" description="Murine stem cell virus, forward primer. Also called MSCV"><BindingSite location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite><BindingSite simplified="1" location="1334-1356" boundStrand="0" annealedBases="CCCTTGAACCTCCTCGTTCGACC" meltingTemperature="62"><Component hybridizedRange="1334-1356" bases="CCCTTGAACCTCCTCGTTCGACC"/></BindingSite></Primer><Primer recentID="14" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="5930-5949" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="5930-5949" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="5930-5949" boundStrand="0" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="5930-5949" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>e33be0fb-1ce0-4b8d-9181-3c104aba3ee5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-IRES-GFP II (pMIG II)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="10:20:38">2025.3.31</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference title="Generation of T-cell receptor retrogenic mice." pubMedID="17406263" journal="Nat Protoc. 2006;1(1):406-17." authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA"/>
</References>
</Notes>
  ý7zXZ  æÖ´F !   t/å£á¼�Ï] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷Ànæìé:¬ñwYE?Ù,<H´ÏwÂ�hMs#/›nëh¿©ÈäÊÏ„“@p­ÞØûÑâpI¡T‹•vØäÊx•ãê!vÅeîpøÍû…��(PfÚ¸,ˆ×-ùÔå	žâšÛóô¼@moî>:?jàå`Ú
m¶ÇËªÖ÷¾·##÷¨Ê%íÃÄ(gì¼Ö»�¹”ÌG™Ã9òÜë_¦
×¨
ìCx¡nr®o	¸³NÙçï÷´ñW‰›ûU	yèê�ÇãÖÅç/}*RËE¦H�×‚ˆ­Þè8ëHŸïà:`SMèë³6æWH.·R Ö¸^¥þ0KÌxƒi‰cŠèoè¢¡žÝ<<	Ù¹aò:}5hÎžÑm2L'vãû^¹ïU{–UäPd½ºj�i“y7fß&k0öí£!ýîõ±ÊÔ©ü6çåí(*ŒâC‰­hª`k÷&yË²eXžþ†C˜Ï)PüåŒ}d¸•'tô`ý5z'I0`
Y¿ÈÆƒ)âxý <Ïˆàjev¡ �L@mù‘ÇB/7œrÃý·d®ç©˜+òa'ÍL*yä”T®ÕdÒ!†±÷;ÀG‘±Ö“ëÇ
Q%™õþÁ6!ôMC>ž�–ô‘eš‹^¸äB	Z~†Š©­®Uo„îd±ó¸!óú÷¸l8ñƒ úÔµÖ}au¦XmDï ² ú=5¿ë¸Ý‹ºµc<QC	Rcî:'ü`Yé4�²î¼èÅkç3Ã‰#‡jÂ{Éµ’
Â¸Ã›þòºÓGxðåÝè90¦Þ¦ïRƒô´'Ië¸~tWB=;‹�ÏJÂPæ5$®�÷ïYXTWN�¾-Pw¥%ÎI»¼”@´c ;®å?çApU‡üíå•6[¼‹Õþ¥·][Ñì©Û½§�B‡¶ÒÍ7R*‚…ýt]’÷cƒŒ7ÍÖŒçÚÃàÌ{„òMIMTå]ZÉ;æâ?E>^_a,òÖhÝ†šCµ¦Ì�0.$§æR2ŠíUòûçËš‹ �ƒ°ønÛ1õÇ¦Î£ªS‘"ÐË[—È�1À�cœ3QQÆ…¡b{±÷1O=BŒð9¿²+E¹l]\1}í¹]é‹.Ï™ãŽíp½ž™+ÐXï(ýAïÉ )š	Ûçq*ÕO%DÕÁˆ_b¸]©ÚZ”9$L‡Ü²ŸòçlÇkÎ»<ÆL„B#¤ï²Î]ˆ¯ 9Éà	Ô�ÖÀÎw8q3ï1)ÍréèÝø[òµª«—¯a ­M·ÏêÐõDU¨ÿ<â®Í| HÉ@¡Z "Ëƒà¶
A@÷Áó+½<:Œ.]2r'püx€pÈ·Ý„ºE8ÒRê”í"ð„°z1Mýjƒ+¥¾-«›�*÷•½ >_¸üaM€Cþen®\a~/Ÿ»|Õâk�QEÁIdÅM$Ÿ'Åñ›û¿íŒ³ØÿðîŸ­¦‚/º^ã,qº—Ü�[;_L5¿q�a›ä1${IÌÑCE&1:Ù!0aµëáû÷
jí›µyQRô§iî†Nå|sñKí•øÄ3_J˜_Rru(‰C¬ qx”N`€•  yöÉè�°¾ç°FE˜V?%M}8yCz›²H`Ä0?»È•<I•J0ìÍ
 LhNàÞI¯©J®½Éj{‘Œ¿”õä¨M•3–i)–³4ß¨2bÉWOÈùw¹ÿ÷BL—$l4KP�Zek‘î[Ô†ä>üŸ¶º6v×qWNÅ/ŠÚÕáU•*ÕŒ›Uz"7¾qYÐ9(${f“·2"°³ú™¢ïGšŒþip×€xlÔD�BÅEµs‡.5‡¯þhÓT qp)Övï™²â²TQ­cÆ’¡è™ù|qÅ€º-D�6¡¡�oÊRfo\†PŒâI®(< žQ*Šm ZÅð.Ò×’ëØÖïÌÐÖ<i=F`?/r³¾„6µç`PŽ­Ì·ªx`_o¯³„hù¢VCd fƒ˜D12dŸŸíVcEä=í‰˜à Ö�q�.ùzõÇ¦T�8`ô…I�2F3%T•n	o÷¹/ú‹'zm�.JGÂ¶Ý—æ»=ñªºzQJt+V+Äpž\RìâlÞì·tKžÈ§�ì‚âž†\ƒRÑ+îyÏËŽˆ�O�Ž&i&�A¡¼\zÎÜ³±Kck�ÂÀ“È¥r™ÃdlíÀ‰;Çä‚:èÄŠá„J<}8I�Q­‡ìÖè-ð³%¥ÿé>RL,2ÒÁlJ£Z- GxWžÌÏ¿æO‹/p &pC4`± Ÿ2òæ.×Ð'ª2³Íñ¯æ´Þ,7Ñž†C¶²0ÛnFn�®$»{±y·¿±Þ2Q+XvõÙúvŸô»#È|˜ãÆ@ Ð€èù(	Ku"x+Y€Œ�ü€¾m›Ký˜DR¶dÀp�d‡uM«€¯ g‚)¼8·LZkþx|GÍ<�\á±×I¿ �é¦f^»½™Ãzköþµ*øT�]0`SwW;õk¹È‡«1vG�L©ðãE–&‡ÊEµÞ\žl#�‘É�3Cð€&)Ûi�rÎfùúÅÁ™áPdßèV¹ˆr ¢(¼g²t¸s¼t›r(O+¦«GxŒ$âEWuWµ» º2€�+(DŽH©¨¢cUOì•z§¦Ý½¤3ðõQ"¬(<ù´×8Ä½äÖ|½Mº¸Sº4ž';U%Š’©U'=UÉ	Ò³’„÷[òp?ÿG×c†ÈÓ®ŽZÆ¾ÕÖEˆŠnÊN€B!½PÈLÈÙ°˜-ÎiYùi+%C„“ ¯½0­ßÜB/¼j}/ºhÓÑ½"¥Z}9è`“ÏØ…?´ýêÓT›>Â-™µ[mióm>2þÞÓe›’êÏÙyHüV¹™¨ÅÒÛöÙwÖë_¤'Å"EÆ�×#“îÞVõÊ*ÝGqíËDg!
·ÃN±+<AxT:Ñˆæ>Z{f�)T\®]ï²Ÿ!`§ë¬þî²¯ª±ú’žN$Ið¬¡šÕý�9à·ZÒïªVœŒ¹‹²{SBh¹#;RP±«µädpD_«x�ˆûWF®|™ûµ;n‡)È3{N
à4À²6ôRËšçìM‰ù`öïeø¯ÛÇ%mø¹]¯›xÒÇãÖ¥Å2ÌÑg‰ÒâOK_c“ŽåBNa¥TÜ4„‹%�ÕÞµî„æ.Ü)ôœŸ5¸�J5#íã�²BrÛéÖADKñÚlÌ?!Rë¼ZÔ¥ýÇæÐ‡o´ÈÒgý/­qí»´Ce®È[Žýý 4(F\0 ¦·^  GÁsZ²bQ8ƒÊõã×NPîÎ”—x¾+²^:fø™ÍM¼^ÔÙñ±#+¯’÷HVn0sQ´½aU�v¨¡ì€ ö	aìžý„áµyU
Wh,wïÜCXIÎ$ÎÔY™•E—þi×Š/¿­Ø++øôF}Ne�¥­�yTIÂ™À{[\)”ùFw†–æ››œxÕu÷‡ =ƒö˜¯lÖø0ÿšÖKÞ`Æ>+Ç¿›¼'0|[F'J\çU�¨‘-é¢}‘ZäXàþÞ»ÊA€N&Â� G
ìíÅ —Lž	ìÑŒK­¤ßÔŠÛsÐØ›.Æq6�‘?G[ñ@¥B³fåáIp�5p]ôvÀý'q›c¾GWK©i€ÎìéYÒqš W¶ŽŒ–„©‘„•IÛs+Ú´*'~–€ÀÆ^ÙU:–‚v;:˜ñG#oÓ7<†s:}ói=îÌpá£i’oä‘À¬|[SLÛBž@è–1 ´Š!�ÍfFØG‡Qzb‚å^Q02-u½.3nZ³éF9Áxf7Sx:Ñx¡|4è5Ñ�Wt•W0˜óþóä…·;ûÕÁ˜Ö&«Š;~õ%er‘8 ¼ˆú>;²ºMÏOñÏuÝ «Î`�¤xó¡þæ×GQÐÒˆ+‘†ÿ’8\Ÿ¥s•ùGÜÂý–e,àÆé­ÑÝÚmÔ6¹ÅZ8e°—Âb,\Ô§ÌŠ®‘‡#¾"¦”JƒéèxtÐåräÆÉÆÜÀr£ìÜ$$ŽPùì&éÁüR¼¤Ðð'æa‘F�6Ãv‚]m»Î›~§ K±UÅ¾¬Þè%ËTS­Ð°¬Q’©¡?+|ëº9L³¿¯Im}÷×÷ÐmÛ¦ÕüÆŒOýt§ú3£\ –¸Áº) ¦tMâü¾˜+<ªç½>÷þU£�ÿXgv?ñ #•ûœ Íºx{Ftp@íŠÇœ¼vÍòÂ´oÕÍ�™ÓEwTYt‹)cÔW½ÞX+Ïx~3;�7yÆõ‹:–j½¼ DøAúoé��ù¢‰g,lvúÝ;-}õÿ¼G¼>Ï
ˆ�£âÌàÃ¡¥…8­xS:|û°>¸<}%œY³íÒˆ6<.¥š´I0‡Ö¤>½~–ÀŠ˜BÊ¼’{Õ!$¢�rEnžÑ©¢jÔ‚å¦‰+&õÚ4®zŠd…J!¤{8JÖ¾ñMÁÛ“jfªÞÕg»„‘êãøË’ü]ð=ZHÅª¹aíåq‡QÛÆè$F¨§MÓU�€©6Ì•¯ŠÇ$c]Wv‹˜”¥ÞÝÜYùa�‚õâöªI8AEZG(±7(Ðs¹¦~d’Óó+žb¬šß $îŠ{Ÿƒït5S7¹†ÊN¦+‡=7´-Êé{~ëÄýw›‚ü3ØWã9p­„Ò,¤ Eµ2ûëRR¼¢U«mD÷[ð~¾ñ6$‡K°�µõ~á—Þöpú;’+¥‹æ¦ÒxúÝ{›áå,Ö¨cï§áÇØ[mx.]	‡†µ_†ÃPpGŽ¯úí)ùƒäñ6K†¼k5=LmÃn%ÉDÞ™áËYôkiY‰ôÊÐà&2õQåEïAq  „y€Á¯¾$*µ†£ƒÊo³PP¤œ‡Ú ·6}Þ˜d-A`ÛÌŒLÈQL”ýw´dU~¬^ü@^oÅé‚{¶�
|þíäAîÑãNW¯J\œ�SÉæ<¬}MzXð€µU(é»Cû$äc?>Ïí¨Ü
„G�ŒÞ‰gdi€ƒõEuM!¶ýj0â«eIøß�
™³šnQÒ›½Ô~š"/çg(ç_w#§Á^\{°2?HÂqŽ{R�ÜÀÓŒWÍÌAàÈT(ÀíŠRZÈÕÊÐÝ:ï_íÌ„ý¸ëÈé[—¢çw0yÒP8K^”O{f³iÙTíÔE«žˆã¨ô»ÂDQ˜³ÿÕ�Ú¸	5²æÞŒZønŸ:öC“’wczÆûÑÉ>TÂÄ=�ëüÓ‚~§t‚IÎ7ØÏd‚¸…Í+éþ¼ñ(K„HÆ\¬�%.ŸÍîçhŽŽyE Ò#”1õEçJÝ—«å0~ÖH©g9Ä; í¶’9ÖDbµÁ!Å¡ü(WƒIV\.ê¸ÇÙJÙ–O¦î¬«ãÚ�³t±†$Ùšev£‡ñÂ€!UÆ[ŠÁ„Ø6¶¯lr•&Ö;Ì7q^M¨Ï±Ü‰WãÁÀœ¸¡V†QF™gñ<9!Ý¨5W4wÄæ‘©Àè¾‚K§°Ž,[š E¯¼[¡ô@6ŠÚiÒþû‚@jÝ¯4A×£Ãž~ê†‡)J¶ÌN¹ø•¹kXàb/{ŠC	àhöîFÜ³+”/ÉoõZý9'�»Ï…�Q3ài šµ0á©ê°QË©A ¬I|$†|éÓMŠ!àH®o^Á]qÄ­Á†qŒJÚÿú,ŒÔ_ÎA‹vy‡ÙkãuÌc¿jYWÓÌß†ÝK‚w,àus¢jÕ³¼Hþ}ôuÆeaŸì|Žvû5VóÖ6jIèãà¼#D_™#|¸ýüž„:ãgµtÉ®ÝæŒ�$þm�wU|e¥‚KÜÙ|l@\x\u÷sìn¨4?ÂlG�	¸ØÁ,…˜ÉJ…vÏÙÅ~�¦F±�–­RóñCèþçkœÓsë|èe<‡šEO	hêµ•Üí�·»ÝW¾ÍgŸˆ§6…ß¡´nR R2í¨ÓcLî’‡¬ûYê@ÔD:Qš¶d‚½Ï¿g�V˜f?Ô£×¹‚óÛ¶îvè�fGå›ÁÚŠº®9xÏÎ¢)xáM¦Õ:9{MÕR¦z»gÕ¯:ÌR o¯8[D^6Umy_¬ÊÞh¬¹xñ«µÿ“e‚åí]$ÅÙŽ(À¸Àõc:AËST�C®B™ä}�ƒ?^]ûZyÜâ}l“Jª`Þó.NŽ£	»\þW"�Œ¿ÿ>
�²6Ù°rcVaç¦
Äç‹„A•7-Üj¯äÔUÊ°Ô	ÈìXÄ`�À0ãFŠ3×+ÿÅyI¯­Áv¦Wqcœ­‹ú˜�ã´sÛ{=óC¤„pùoiñ¶¤¼È°±N5ÔÖ®¨•¹AFc*®?ûdÓ«ÚôJªXÙùÂß–¢Zº\Ø´ªo¦vó<~ ,ßéPi€yÑìå^Òa\c
šÈ•áàlŒ	ñ„äá¢cØøºÚ$�m™ƒYkƒ+Êíôd9�€nMœå)¤'vV5ë™tßI
Ø§‰á¦Ô·l¤4¡z90!½€÷Ù{ENa£ReÑg>œb%@‰1�6O!½p¶OH=êô•lû	†×j*ˆÕy@¿@×ÇÒ4ÄŽŒîûmÐÒ÷ïÞ‡äµó»fŸáž¸÷7ÔY�iàwµ¤Ž%šiä)¦‡
ÅŽRy¤òTûë(ãVÒ /Åöâ°’ä×&5¼Jâ*xÍ}ÃÁÐ ¡$g³ÀfaQÐÎÜ8Â<ËÃ¶5×¤!arÙßÇÐzÊ[s¸´ñø5šà:ïöìtj;ã3~�êÉžÃVíC÷?*Ý®ºmœL|‡Ú ô‚Ok;TV•]Î ¾ÞmN‹XP=.?^2ž.�5PA£6Ù¥o¬º2¬²Ôî³Š³×ÿÐWÝ¹,„‡Ø`µ7TµW±E94qË‹K§CIåÁôÒ¹„-mÇÝ|Ødgpûç¥Ç¯ë¤¾Äâ9; §JFK› ŸcvªÆ@=�7#š±§ŒKà3=Ö›WI�ûsìN~Âúðæ°+Ó£8Ú'ç2˜¤Å|{âðmÚ™Š¤]Rnäy�GMæ9‹åÐ%v!¯�w¶ÑÙqX£<åA¶ > %®êRYµ‰«Ö×äç1HþR'ˆ¬ [Ñyó"ž‹!:h¶åÜºF†¥±ß§Z…²Ñ«®ŽpAF]Cùˆ†F:~Ù—º»pd(s2
nà„†ö#ã	4‘-Øø¿–´a15c¹èŒš…ùnªU*Ì8lnÌY äãâ=×€i¬e"4+…è¢/J/‡30ãöÝ1ˆøÖ¡¾À�ÕÄW­ýÿÛ¡ö�ëûµÌc�ÉÈ¸Å[§álÝ&0´z-8®ÆÀ­�¹qí¢UÀ}¿FÐjûvv_ˆê%kuŠ) ðš˜ãR-:ÜÚÑóÃ(ï,¸´êmvµ�mãLwP	®�èžz¾+Ö­"¡\wÿÎ‡5Viøû¼¨±½IQÝ1¯�.ÀŠ’¤ý..Y"­Å<D67šä³co×ÔÝ.M    ¿ÊâX¾ ë-Žù øúNg±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  (U<?xml version="1.0"?><Features nextValidID="15"><Feature recentID="0" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1443-1451" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="1" name="gag (truncated)" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="989-1405" color="#993366" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated Moloney murine leukemia virus (MMLV)&amp;nbsp;&lt;i&gt;gag&lt;/i&gt; gene lacking the start codon&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="2" name="MESV Î¨" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="581-922" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="3' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7467-7981" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;3' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="5' LTR" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-517" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;5' long terminal repeat from murine embryonic stem cell virus&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="7305" maxFusedRunOn="7305" detectionMode="exactProteinMatch"><Segment range="6586-6588" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="6589-6591" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6592-7302" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"/></Q></Feature><Feature recentID="6" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="6586-6588" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="6589-6591" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="6592-7305" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="7" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8521-9109" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8143-8159" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8212-8233" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="8167-8173" color="#ffffff" type="standard"/><Segment range="8174-8191" color="#ffffff" type="standard"/><Segment name="-35" range="8192-8197" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="10071" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="9280-10071" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="10072-10140" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="12" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="10141-10245" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="Myc" directionality="1" translationMW="1203.34" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="6002" maxFusedRunOn="6002" detectionMode="exactProteinMatch"><Segment range="1452-1481" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Myc (human c-Myc oncogene) epitope tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EQKLISEEDL"/></Q></Feature><Feature recentID="14" name="Dcaf1-m" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1482-6002" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDRENFRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGSHATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLLEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEEGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  l<Notes>
<UUID>f3f452b3-3b55-4cf2-95d8-a7da8a8ff5e5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMSCV-Myc-Dcaf1(mouse)-IRES-EGFP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP.</Description>
<Created UTC="20:21:22">2021.5.12</Created>
<LastModified UTC="5:43:53">2026.5.8</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference pubMedID="17406263" volume="1(1)" journalName="Nat Protoc." authors="Holst J, Szymczak-Workman AL, Vignali KM, Burton AR, Workman CJ, Vignali DA" journal="Nat Protoc. 2006;1(1):406-17." date="2006" pages="406-17" title="Generation of T-cell receptor retrogenic mice." type="Journal Article"/>
</References>
</Notes>
  Y     –         
?????                                                                                                       ÿ d      DO  d              E              ÿÿÿÿYô? P                                                                                                                                                             <StrandColors><TopStrand><ColorRange range="1404..1435" colors="black"/><ColorRange range="1436..1480" colors="light blue"/></TopStrand><BottomStrand><ColorRange range="1404..1435" colors="black"/><ColorRange range="1436..1480" colors="light blue"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
